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GW23-e1735
SYNERGISTIC INDUCTION OF ADIPONECTIN GENE EXPRESSION AND SECRETION BY ENDOTHELIN-1 AND ANGIOTENSIN II IN CARDIOMYOCYTE
  1. Bingyan Guo1,
  2. Derong Han1,
  3. Yongjun Li2
  1. 1Hebei Gucheng County Hospital
  2. 2The Second Affiliated Hospital of Hebei Medical University

    Abstract

    Objectives We have demonstrated previously that angiotensin (Ang) II may upregulate adiponectin expression in hypertrophic cardiomyocytes via the angiotensin type-2 receptor/nitric oxide/cGMP signalling pathway. In this study, we further examined the combined effect of endothelin (ET)-1 and Ang II on adiponectin gene expression and secretion.

    Methods neonatal rat ventricular myocytes (NRVMs) were treated with various concentrations of Ang II and (or) ET-1, adiponectin expression was measured by qPCR. Adiponectin release was measured by enzyme-linked immuosorbent assay.

    Results ET-1 and Ang II induced adiponectin release in a synergistic manner that can be attributed to their synergistic induction of adiponectin gene expression, as evidenced by adiponectin mRNA analysis. Both ETA and ETB receptors seem to be involved. A nitric oxide synthase inhibitor (Nx-nitro-Larginine methyl ester hydrochloride) and an analogue of cGMP antagonist (Rp-8-Br-CGMP-S) also partly blocked ET-1 -mediated up regulation of adiponectin.

    Conclusions ET-1 and Ang II may boost adiponectin secretion in a synergistic manner, probably through their synergistic induction of adiponectin gene expression in NRVMs. A commom mechanism via the nitric oxide/cGMP/protein kinase G signalling pathway may be involved.

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