Article Text

PDF

GW23-e2234
IN-STENT LIPID-RICH PLAQUE IN HIGH CHOLESTEROL DIET RABBIT VALIDATED BY INTRAVASCULAR OPTICAL COHERENCE TOMOGRAPHY AND HISTOLOGY
  1. Yan Fang,
  2. Jian Shi,
  3. Zulong Xie,
  4. Linna Zhang,
  5. Jingbo Hou,
  6. Bo Yu
  1. Department of Cardiology, Second Affiliated Hospital of Harbin Medical University, Harbin, China

    Abstract

    Objectives In-stent lipid-rich plaque had been found frequently in patients underwent stent implantation by optical coherence tomography (OCT) and intravascular ultrasound (IVUS). The paucity of a reliable animal model limits the further research of lipid-rich plaque development and treatment. The present study aims to develop an animal model of lipid-rich plaque.

    Methods Four New Zealand White Rabbits were fed a high cholesterol diet (HCD) until euthanasia. Eight bare metal stents were randomly implanted into iliac arteries after 1 week HCD. The rabbits underwent OCT and IVUS imaging at 8 weeks after stent implantation. Then the stents were harvested and processed for light microscopy. On OCT imaging, in-stent lipid-rich plaques were identified by diffusely bordered, signal-poor regions. In-stent plaques were defined as peri-strut fomay macrophage clusters with or without calcification, fibroatheroma, and ruptures with thrombosis in in-stent neointima.

    Results All stents underwent OCT and histology examination. With OCT, total 127cross-sections and 962 struts were analysed. The 99.75% struts were covered well at 8 weeks after implantation. In-stent lipid-rich plaques with diffusely bordered, signal-poor regions were identified in 23.40% of cross-sections and 87.50% of stents. The histology examination of corresponding images further confirmed the components of in-stent lipid-rich plaque. In-stent lipid-rich plaques were characterised by peri-strut fomay macrophage clusters with lipid-rich necrotic core in in-stent neointima.

    Conclusions In-stent lipid-rich plaque model was developed successfully in high cholesterol diet rabbit. OCT is a useful tool to detect in-stent lipid-rich plaque features in vivo. The combination of in vivo OCT and the in-stent lipid-rich plaque model may be an important research tool in furthering our understanding and treatment of in-stent plaque in patients underwent stent implantation.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.