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  1. Ying Qing Li1,
  2. Hong Yan Wen2,
  3. Chun Lin Hu1,
  4. Hong Yan Wei1,
  5. Chen Mu Ai2,
  6. Gang Wang2,
  7. Xiao Xing Liao1,
  8. Rong Liu1,
  9. Xin Li1
  1. 1Department of Emergency, The First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan Road II, Guangzhou, People's Republic of China
  2. 2Department of Cardiology, The First Affiliated Hospital, NanHua University, 69 ChuanShan Road, Hengyang, People's Republic of China


    Objectives Recent studies have shown that circulating microRNAs (miRNAs) might be useful novel biomarkers for the diagnosis of acute myocardial infarction (AMI). The aim of this study is to evaluate the expression of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) in AMI and compare their diagnostic values with that of cardiac troponin T (cTnT).

    Methods Sixty-seven plasma samples obtained from patients with AMI and thirty-two plasma specimens collected from healthy volunteers were analysed in this study. The levels of cardiac-specific miRNAs (miR-1, -133a, -208b, and -499) were measured by quantitative reverse transcription-PCR (qRT-PCR), and the concentrations of plasma cTnT were measured using electrochemiluminescence-based methods on the Elecsys 2010 Immunoassay Analyzer.

    Results The levels of plasma miR-1, -133a, -208b, and -499 were significantly increased in AMI patients (p<0.001) compared with healthy volunteers. The expression of the cardiac-specific miRNAs in AMI patients decreased to close to the baseline at the time of hospital discharge (p>0.05). There was no correlation between the levels of the four circulating miRNAs and the clinical characteristics of the study population (p>0.05). Furthermore, receiver operating characteristic (ROC) curve analyses showed that the four plasma miRNAs were not superior to cTnT for the diagnosis of AMI.

    Conclusions Our results demonstrated that the circulating miRNAs miR-1, -133a, -208b, and -499 may be useful biomarkers for AMI but are not superior to cTnT for the diagnosis of AMI.

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