Objectives Whether ultrasound mediated microbubbles could enhance the product by non-enzymatic pathway will be discussed in this chapter.
Methods Mix Laevo-arginine (L-Arg) with hydrogen peroxide (H2O2) by the concentration rates 1:1, 10:1, 10:0.1, 1:10, irradiates with ultrasound for 60 s. (frequency: 1MHz, output power: 0.5, 1, 1.5 W/cm2), and test the formation of nitric oxide (NO).
Results The experimental group generates the most NO of all groups, ultrasound group was better than micro bubbles group and blank control group, mcirobubbles group and blank control are normal. Besides, NO product has positive relation with output power in local range.
Conclusions Ultrasound mediated microbubbles could enhance NO product by non-enzymatic pathway.