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GW23-e1137
EXPRESSION OF LYMPHOCYTE KCA3.1 AND CYTOKINE IN SHR
  1. Wang Ling-peng,
  2. Luo Jian
  1. Department of cardiology of the First Affiliated Hospital, Xin Jiang Medical University

    Abstract

    Objectives to research the expression of intermediate-conductance Ca2+-activated K+ channel (KCa3.1), TNF-α mRNA and protein in lymphocyte derived from spontaneously hypertensive rat (SHR).

    Methods Take SHR and Wistar rats as experimental animals, to separate peripheral blood lymphocytes in rats, using Real-time PCR and Western blot technique were used to detect the express of KCa3.1, TNF-α in SHR lymphocytes.

    Results (1) In SHR, the expression of KCa3.1 gene was significantly higher in lymphocytes(1.3025±0.2117 vs 0.4475±0.2012; p<0.05) compared with Wistar rats. The expression levels of TNF-α mRNA in the SHR lymphocytes were significantly increased compared with the control group (1.4257±0.1317 vs 0.3836±0.1626; p<0.05).(2) KCa3.1, TNF-α protein expression were also increased in SHR than in control(p<0.05).

    Conclusions The lymphocyte KCa3.1, TNF-α expression are upregulated in SHR suggesting KCa channel may contribute to the development of hypertension by lymphocyte activation.

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