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GW23-e0222
OX40-OX40L INTERACTION TARGETS NFATC1 IN APOE−/− MICE DURING ATHEROGENESIS
  1. Jinchuan Yan,
  2. Liangjie Xu,
  3. Yuan wei,
  4. Wang cuiping,
  5. jinchuan yan
  1. The Affiliated Hospital of Jiangsu University

    Abstract

    Objectives We investigated the effect of OX40-OX40L interaction on the nuclear factor of activated T cells c1 (NFATc1) in ApoE−/− mice.

    Methods Atherosclerotic plaque was induced by rapid perivascular carotid collar placement in ApoE−/− mice. The expression of OX40, OX40L and NFATc1 in lymphocytes was measured by Real Time PCR (RT-PCR) and flow cytometry (FCM), respectively. The presence of NFATc1 in the atherosclerotic plaque was detected by immunohistochemistry. The level of IL-4 was measured by ELISA.

    Results We found that the expression of NFATc1 was significantly elevated both in atherosclerotic lesion and in leukocytes from ApoE−/− mice. In vitro, after stimulating OX40-OX40L interaction by anti-OX40L mAb, NFATc1 mRNA expression and protein concentration increased by RT-PCR and FCM, respectively in lymphocytes, while anti-OX40L mAb suppressed expression of NFATc1 in lymphocytes. Moreover substantially elevated level of IL-4 was induced by anti-OX40 mAb, while NFATc1 inhibitor markedly suppressed production of IL-4.

    Conclusions This study suggests that OX40-OX40L interaction regulates the expression of NFATc1, which may play a critical role in atherosclerotic plaque formation, which might also have implications with pathophysiology of atherosclerosis.

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