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GW23-e1271
5-AMINOLEVULINIC ACID-DERIVED PROTOPORPHYRIN IX MEDIATED FLUORESCENCE DIAGNOSIS AND PHOTODYNAMIC THERAPY OF MACROPHAGES WITHIN THE ATHEROSCLEROTIC PLAQUE
  1. Haibo Chen,
  2. Jianting Yao,
  3. Xin Sun,
  4. Ye Tian
  1. Department of Cardiology, The First Affiliated Hospital, Harbin Medical University

    Abstract

    Objectives To detect the accumulation of ALA-PpIX in the plaque macrophages and evaluate the effects of PDT on macrophages.

    Methods The fluorescence of PpIX was detected by fluorescence microscope in THP-1 macrophages and plaque fresh-frozen sections of rabbit carotid artery. Immunofluorescent staining of plaque macrophages and SMCs was performed to locate ALA-PpIX. Plaque burden was evaluated by Haematoxylin and eosin (H&E) staining. Plaque macrophages and SMCs content was determined by immunohistochemical staining.

    Results PpIX was found accumulated in the cytoplasm of THP-1 macrophages. In the plaque fresh-frozen sections, the fluorescence of ALA-PpIX was detected where macrophages immunofluorescence staining was positive. Compared with the control group, the plaque area was reduced by 59% p<0.01) at 4 week after PDT, the plaque macrophages content decreased by 56% p<0.001) at 1 week and 64% p<0.001) at 4 week respectively.

    Conclusions ALA-PpIX is preferentially accumulated in the macrophages of plaque and ALA-PpIX mediated PDT significantly decreases macrophages content, indicating a promising strategy of atherosclerotic plaque macrophages diagnosis and therapy.

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