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GW23-e0619
HEPATOCYTE GROWTH FACTOR GENETICALLY MODIFIED BONE MARROW-DERIVED MESENCHYMAL STEM CELLS TRANSPLANTATION PROMOTES ANGIOGENESIS IN A RAT MODEL OF HINDLIMB ISCHAEMIA
  1. Guanhua Su,
  2. Yufei Sun,
  3. Yongxin Lu,
  4. Xinxin Shuai,
  5. Yuhua Liao,
  6. Qiyun Liu,
  7. ping Luo,
  8. Yongxin Lu
  1. Department of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China

    Abstract

    Objectives Angiogenic gene therapy and cell-based therapy for peripheral arterial disease (PAD) have been studied intensively currently. This study aimed to investigate a new strategy whether combining mesenchymal stem cells (MSCs) transplantation with ex vivo human hepatocyte growth factor (HGF) gene transfer was more therapeutically efficient than the MSCs therapy alone in a rat model of hindlimb ischaemia.

    Methods One-week after establishing hindlimb ischaemia models, Sprague-Dawley rats were randomised to receive HGF gene-modified MSCs transplantation (HGF-MSC group), untreated MSCs transplantation (MSC group), or PBS injection (PBS group), respectively.

    Results Three-weeks after injection, angiogenesis was significantly induced by both MSCs and HGF-MSCs transplantation, and capillary density was the highest in the HGF-MSC group. The number of transplanted cell-derived endothelial cells was greater in HGF-MSC group than in MSC group after 1 week treatment. The expression of angiogenic cytokines such as HGF and VEGF in local ischaemic muscles was more abundant in HGF-MSC group than in the other two groups. In vitro, the conditioned media obtained from HGF-MSCs cultures presented proproliferative and promigratory effects on endothelial cells.

    Conclusions HGF gene-modified MSCs transplantation therapy may induce more potent angiogenesis than the MSCs therapy alone. Engraftment of MSCs combined with angiogenic gene delivery maybe a promising therapeutic strategy for the treatment of severe PAD.

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