Article Text

PDF

GW23-e1609
THE EFFECT OF OXIDISED LOW-DENSITY LIPOPROTEIN ON NOTCH1 EXPRESSION IN MACROPHAGES
  1. Wang Yuqin1,
  2. Li Ming2
  1. 1The PLA252Hospital Department of Cardiology, BaoDing 071000, China
  2. 2Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of science and Technology, Wuhan

    Abstract

    Objectives To explore the expression of Notch 1 by ox-LDL in Macrophages.

    Methods Human macrophage from THP1 cell line transform by PMA was cultured with different concentration of ox-LDL for 48 h to induce proinflammatory response. Macrophages were harvested and supernatants were collected for further experiments. The expression of Notch1 mRNA and protein were measured by real-time quantitative PCR (RT-PCR) and Western blot, respectively. The levels of vascular cell adhesive molecule-1 (VCAM-1) and Monocyte chemoattractant protein-1 (MCP-1) were determined by RT-PCR and ELISA, respectively.

    Results Our data showed that with ox-LDL challenge, The expressions of Notch1 and the levels of VCAM-1 and MCP-1 significantly increased in macrophages in a dose-dependent manner within some extent compared with that in the control group p<0.05).

    Conclusions Notch signalling is activated by ox-LDL stimulation and regulates macrophage functions.

    Statistics from Altmetric.com

    Request permissions

    If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.