Objective To investigate the effects of monocyte chemoattractant protein-1 (MCP-1) A-2518G genetic polymorphism and its serum levels on acute myocardial infarction (AMI) in Chinese Han population of Sunan region.
Methods The A-2518G polymorphism of MCP-1 gene was genotyped in 338 patients with AMI and 192 control subjects by polymerase chain reaction and restriction fragment length polymorphism analysis and DNA sequencing; Serum concentration of MCP-1 was randomly measured in 94 patients with AMI and 73 control subjects by enzyme-linked immune sorbent assay.
Results Significant difference was found neither in genotype distribution nor in allele frequencies of MCP-1 gene A-2518G between AMI and controls (GG:31.36%, AG:52.66%, AA:15.98% in AMI; GG:34.37%, AG:53.65%, AA:11.98% in controls (P = 0.428), the G allele prevalence was 0.5769 in AMI group and 0.6120 in control subjects (P = 0.270). Multivariate logistic regression analysis revealed that MCP-1 gene –2518G allele was not associated with increased risk of AMI [χ2 = 0.408; P = 0.523; OR: 0.800 (0.404–1.585) ]. The serum level (M/IQR) of MCP-1 was significantly higher in AMI group (186.24/285.15) pg/ml than that in controls (100.71/134.02) pg/ml (P = 0.001). Multiple linear regression analysis further revealed that the serum levels of MCP-1 was associated with hypertension, diabetes mellitus, smoking and male, but not with MCP-1 gene –2518G allele in AMI group. Multivariate logistic regression analysis revealed an elevated serum level of MCP-1 was not associated with increased risk of AMI [χ2 = 2.665; P = 0.103; OR:2.782 (0.814–9.505) ].
Conclusions Neither the MCP-1 gene A-2518G polymorphism nor its serum levels was associated with increased risk of AMI, and the MCP-1 gene A-2518G polymorphism does not yet effect its serum levels in Chinese Han population of Sunan region.