Objectives To investigate and compare the ability of stem cells from white and brown adipose tissue spontaneous differentiation into cardiomyocytes in vitro, and develop new way to heart regeneration.
Methods The differentiated cardiomyocyte-like cells were identified bymorphological observation, confirmed with expression of specific cardiac genes, immunofluorescence and ultrastructural analysis. Electrophysiological studieshave shown the pacemaker activity of the differentiated cells.
Results Here, we found that brown adipose-derived stem cells (ASCs) could differentiated into beating cardiomyocytes spontaneously in vitro, but not the white ASCs. Likewise, mature brown adipocytes can trans differentiate into spontaneously beating cardiomyocytes.
Conclusions Our result not only confirmed the brown adipocytes can differentiate into cardiomyocytes spontaneously in vitro but also offered new insights into cardiomyocyte differentiation and regeneration.