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GW24-e0758 New insights into cardiomyocyte differentiation from adipose-derived stem cells, promoting understanding of brown adipocyte
  1. Deng Zijun1,2,
  2. Chen Lei1,
  3. Kang Xinjiang2,
  4. Wang Changhe2,
  5. Chai Zuyin2,
  6. Li Yuquan1,
  7. Zhang Chuansen1,
  8. Zhou Zhuan2,
  9. Yang Xiangqun1
  1. 1Institute of Biomedical Engineering, Second Military Medical University, Shanghai 200433, PR China
  2. 2State Key Laboratory of Membrane Bioengineering and the Center for Life Sciences, Institute of Molecular Medicine, Peking University, Beijing, China

Abstract

Objectives To investigate and compare the ability of stem cells from white and brown adipose tissue spontaneous differentiation into cardiomyocytes in vitro, and develop new way to heart regeneration.

Methods The differentiated cardiomyocyte-like cells were identified bymorphological observation, confirmed with expression of specific cardiac genes, immunofluorescence and ultrastructural analysis. Electrophysiological studieshave shown the pacemaker activity of the differentiated cells.

Results Here, we found that brown adipose-derived stem cells (ASCs) could differentiated into beating cardiomyocytes spontaneously in vitro, but not the white ASCs. Likewise, mature brown adipocytes can trans differentiate into spontaneously beating cardiomyocytes.

Conclusions Our result not only confirmed the brown adipocytes can differentiate into cardiomyocytes spontaneously in vitro but also offered new insights into cardiomyocyte differentiation and regeneration.

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