Article Text

PDF
GW24-e0757 Ginsenosides-Rbl improves adriamycin-induced heart failure by adjusting the protein kinase R-like ER kinase pathway
  1. HongLiang Kong,
  2. Kong Hong-liang
  1. Cardiology Center, The People’s Hospital of LiaoNing Province

Abstract

Objectives Basing on the adriamycin-induced heart failure (HF), the present study elucidated whether the effect of Ginsenosides-Rbl (Gs-Rb1) improving cardiac function was performed by inhibiting protein kinase R-like ER kinase (PERK) pathway.

Methods Adriamycin-induced rats with HF were randomly divided intoHF group (n = 15) and Gs-Rb1 group (n = 17), and the health age-matched rat was as control (n = 15). After the intervention being performed and the left ventricular ejection fraction (LVEF) was analysed by echoeardiography, glucose-regulated protein 78 (GRP78), protein kinase R-like ER kinase (PERK), p-PERK, eukaryotic initiation factor 2α (eIF2α), p-eIF2α, C/EBP homologous protein (CHOP) and caspase-12 were assayed by western bolt and Rt-PCR.

Results

  1. HF was performed by adriamycin, LVEF was significantly improved by Gs-Rb1 (P = 0.000).

  2. Both mRNA and protein of GRP78 were significantly up-regulated in HF group than in control group (P = 0.000), which were significantly down-regulated in Gs-Rb1 group than in HF group (P = 0.000).

  3. Both PERK and p-PERK were significantly inhibited by Gs-Rb1, compared to HF group (P = 0.000).

  4. eIF2α mRNA, eIF2α protein and p-eIF2α protein, being significantly up-regulated by adriamycin, were markedly down-regulated by Gs-Rb1.

  5. Both mRNA and protein of CHOP were significantly higher in HF group than in control group (P = 0.000), which were significantly lower in Gs-Rb1 group than in HF group (P = 0.000).

  6. Both mRNA and protein of caspase-12, being significantly increased in HF group (P = 0.000), were markedly decreased by Gs-Rb1 (P = 0.000).

Conclusions The effect of Gs-Rb1, improving HF, was partly fulfilled through adjusting PERK pathway at least.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.