Article Text

PDF
GW24-e1783 The experimental study of protective effects of Atrial Natriuretic Peptide (ANP) Injection on the cultured human umbilial vein endothelial cells injured by hypoxia-reoxygenation
  1. Chen Zhang Qiang
  1. Jiangxi Provincial People’s Hospital

Abstract

Objectives To study the protective effects of Atrial Natriuretic Peptide (ANP) Injection on the cultured human umbilial vein endothelial cells injured by hypoxia-reoxygenation

Methods hypoxia-reoxygenation group (n = 6): cells were cultured in the hypoxia box for 6 hours, then reoxygenation for 6h; 3. hypoxia-reoxygenation + different concentrations of ANP Group: 0.001 µg/ml (n = 6); 0.005 µg/ml (n = 6); 0.01 µg/ml (n = 6); 0.05µg/ml (n = 6); 0.1µg/ml (n = 6), and also hypoxia cultured for 1 h and reoxygenation 6 h; the cells in the cell morphology was observed under inverted microscope, cell culture supernatant obtained in each group were measured {malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and endothelin (ET) content}, the final results of statistical analysis conducted.

Results The dehydrogenase (LDH) activity in hypoxia-reoxygenation group was significantly higher compared with the control group (69.35 ± 5.66 vs 31.04 ± 3.43, P < 0.01); LDH activity in drug intervention group was significantly decreased compared with hypoxic reoxygenation (P < 0.01). MDA content in hypoxia-reoxygenation group was significantly higher than that in the control group (5.94 ± 0.58 vs1.69 ± 0.16, P < 0.01), but MDA content in drug intervention group was significantly lower than that in hypoxia-reoxygenation group (P < 0.01), during the range of ANP concentration from 0.001 µg/ml to 0.05 µg/ml), MDA content in the culture medium concentration showed negative correlation with ANP concentration in the ANP group (P < 0.01). Levels of intracellular SOD in hypoxia-reoxygenation group were significantly lower than that in the control group (15.74 ± 2.17 vs 47.08 ± 4.23, P < 0.01) and was significantly higher in drug treatment group than in hypoxia-reoxygenation group (P < 0.01), When ANP concentration range from 0.001 µg/ml to 0.05 µg/ml, each sub-group correlation analysis showed medium concentration of SOD content was positively correlated with ANP concentration in ANP intervention group (P < 0.01); Nitric oxide (NO) levels in cell culture medium in hypoxia group was significantly lower than that in the control group (36.81±3.78 vs 89.78±7.01, P <0.01), ET-1 content was significantly higher than that in the control group (1368.64 ± 99.07 vs. 305.52 ± 35.30 P <0.01); Correlation analysis showed that: ANP concentration ranged from 0.001 µg/ml to 0.05 µg/ml, in each sub-group of ANP intervention group, NO levels were positively correlated with ANP (P < 0.01), while the concentration of ET-1 concentration was negatively correlated with ANP concentration (P < 0.01).

Conclusions ANP could reduce the concentration of LDH in culture medium, and has a protective effect on the cell membrane; ANP could reduce the MDA content in the culture medium and increased human umbilical vein endothelial cell SOD content, thus inhibiting the oxidative damage on endothelial cells caused by hypoxia-reoxygenation; ANP improved the content of NO and reduced the content of ET-1 after human umbilical vein endothelial cells undergoing hypoxia-reoxygenation injury, thus played important role in protection of the endothelial function.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.