Objectives gastric mucosal lesion induced by dual antiplatelet (aspirin combined with clopidogrel) is an universal concern of the first and second prevention of Coronary heart disease. This study is to observe the repair effect of panax quinquefolius saponin on gastric mucosal lesion induced by dual antiplatelet drug.
Methods 36 SD rats, ligate left anterior descending coronary artery as experimental Acute Myocardial Infarction (Acute Myocardial Infarction, AMI) model. The successful operation of rats were randomly and equally divided into AMI model group (distilled water 2 ml/kg/d by gavage, 12 rats), dual antiplatelet group (aspirin 9 mg/kg/d and clopidogrel 6.75 mg/kg/d by gavage, 12 rats), dual antiplatelet and PQS group (dual antiplatelet and PQS 162 mg/kg/d by gavage, 12 rats), The other 12 rats were regarded as Sham-operated group (Only puncture not ligation of coronary artery, distilled water 2 ml/kg/d by gavage,12 rats) After duplicating the mould 2nd days, distinguish to infuse by corresponding the soup feeding 28 days. Then under 4% chloral hydrate anaesthesia, Blood plasma were collected by abdominal aortic method, To measure serum gastrin (GAS), nitric oxide (NO), interleukin (IL) 1 β (IL- 1 beta), tumour necrosis factor alpha (TNF alpha) and plasma motilin (MTL), endothelin (ET 1) content, and the changes of histology and ultrastructure of gastric mucosa were observed.
Results Gastric mucosa damage index (Guth score) and pathological grading index of Sham-operated group (Whittle score) and AMI group were zero, Compared with the AMI group, the gastric mucosa Guth score and Whittle score of dual antiplatelet group were significantly increased (P < 0.01). Compared with the dual antiplatelet group, dual antiplatelet and PQS group gastric mucosa congestion reduced, inflammatory cell infiltration reduced, stomach surface cells were relatively complete. The Guth score and Whittle score were significantly decreased (P < 0.01). Compared with the AMI group, dual antiplatelet group rats plasma ET- 1, MTL, serum IL- 1 β, TNF alpha content elevated significantly (P < 0.05), Serum NO and GAS content reduced significantly (P < 0.05). Compared with dual antiplatelet group, dual antiplatelet and PQS group Serum NO and GAS content decreased significantly (P < 0.05), Plasma ET- 1, MTL, serum IL- 1 β, TNF alpha significantly reduced (P < 0.05).
Conclusions PQS has a certain effect for repairing gastric mucosa damage induced by dual antiplatelet. The mechanism may be related to promoting GAS, NO protection factor formation, reducing inflammatory factor IL- 1 β, TNF alpha secretion, reducing ET- 1, MTL damage factor formation.