Article Text

GW24-e2163 Expressions of endothelin-converting enzyme in primary cultured atrial myocytes after rapid electrical-field stimulation
  1. Zhang Po,
  2. Zhu Xianyang
  1. Department of Congenital Heart Disease, Cardiovascular research institute of PLA, General Hospital of Shenyang Military Command, Shenyang City, China


Objectives To study the expressions of endothelin-converting enzyme (ECE2) at early stages of atrial fibrillation in a rapid paced primary cultured atrial myocyte model.

Methods Primary rat atrial myocytes were cultured and a rapid paced cell model was established. The atrial cells were divided into five groups with pacing durations within 0 to 24 h. The polymerase chain reaction (RT-PCR) and Western blot (WB) were applied to detect the messenger ribonucleic acid (mRNA) and proteins expression of ECE2.

Results mRNA expression of ECE2 increased after 3 h of rapid pacing and continued to elevate in a time dependent manner. Similarly, changes of ECE2 protein were paralleled with mRNA expression.

Conclusions Expressions of ECE2 increased in early phase of rapid paced atrial myocytes. It implicated the ECE2 and endothlin-1 was implicated in pathological remodelling of atrial fibrillation.

Statistics from

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.