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GW24-e0042 Effects of AngII and Ang-(1-7) on the expression of SR-BI, ABCA1 and cholesterol efflux in THP-1 derived foam cells
  1. Chai Chanjuan,
  2. Yng Zhiming
  1. Department of Cardiology, The Second Hospital of Shanxi Medical University

Abstract

Objectives To investigate the effects of angiotensinII (AngII) and angiotensin-(1-7) [Ang-(1-7)] on scavenger receptor class B type1 (SR-BI), ATP-binding cassette transporter A1 (ABCA1) and cholesterol efflux in THP-1 derived foam cells.

Methods Human monocytic cell line (THP-1) was induced intomacrophages by100 nmol/L phorbol myristate acetate (PMA) for 48h, and treated with ox-LDL to construct the foam cells, then were randomly allocated into five groups: control group, AngII group,Ang-(1-7) group, Ang-(1-7) + AngII groupand AngII + Ang-(1-7) + A-779 group. Them RNA and protein expression of SR-BI, ABCA1were determinded by RT-PCR and Westernblot, the intracellular cholesterol efflux rate was detected by liquid scintillator.

Results Compared with the control group, AngII decreasedSR-BI andABCA1 in both protein and mRNA, and inhibited the cholesterol efflux (P < 0.05). Those effects could be attenuated by cotreated with Ang-(1-7)(P < 0.05). However when incubated with A-799, an inhibitor of Ang-(1-7), the effects ofAng-(1-7) on promoting the expression of SR-BI, ABCA1 and the cholesterol efflux were significantly abolished (P < 0.05).

Conclusions In THP-1 derived foam cells, Ang-(1-7) via its specific receptor MAS attenuates the reduction of the expression of SR-BI and ABCA1 induced by AngII, and increases the cholesterol efflux.

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