Objectives To investigate the effects of Nε-carboxymethyl-Lysine (CML), a key bioactive components of AGEs, on migration from RAW264.7-derived foam cells.
Methods The transmembrane migration ability was assessed by Boyden chamber cell migration experiment in vitro. The intracellular accumulation of free cholesterol, cholesterol ester and total cholesterol was determined by cholesterol oxidase methods. The expression of CD36 mRNA and protein was analysed by RT-PCR and Westernblot.
Results 10 μmol/L CML significantly promoted the intracellular accumulation of free cholesterol, cholesterol ester and total cholesterol in RAW264.7-derived foam cells, upregulated the expression of CD36 mRNA and protein, inhibited the transmembrane migration ability of foam cells (The mean fluorescence intensity of migrated foam cells in CML + oxLDL group was reduced by 59.79%, compared with oxLDL group; It was reduced by 73.46%, compared with control group.).
Conclusions CML may initiate CD36 cascade by cooperating with oxLDL and result in the inhibition of the transmembrane migration ability.