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GW24-e1903 Effect of ox-LDL and atorvastatin on the production of interleukin-18 in and the proliferation of cultured human umbilical vein endothelial cells
  1. Zhou Aiqin,
  2. Wang Xiaoping
  1. Department of Cardiology, The First Affiliated Hospital of Gannan University, Ganzhou 341000, China

Abstract

Objectives To investigate the effects of atorvastatin on the production of interleukin-18(IL18) in and the proliferation of cultured human umbilical vein endothelial cells (HUVECs) activated by oxidised low density lipoprotein (Ox-LDL).

Methods HUVECs Cultured in vitro at passage 3 to 9 were used for experiment. There were three groups: 1) control group; 2) Ox-LDL groups (100 mg/L); 3) Ox-LDL + atorvastatin groups: cells were firstly incubated with 0.01, 0.05, 0.1, 0.5, 1 µmol/L atorvastatin respectively for 4 h,then incubated with 100 mg/L Ox-LDL for 24 h. IL18 in supernatant medium was detected by cell ELISA and cell proliferation was measured by MTT colorimetric assay.

Results Compared with control group,ox-LDL (100 mg/L) could inhibit the proliferation of HUVECs (P < 0.01). Atorvastatin could dose-dependently enhance the proliferation of HUVECs activated by Ox-LDL (P < 0.05, P < 0.01). HUVECs didn’t release IL18 without stimulation. ox-LDL(100 mg/L) stimulated the production of IL18 by HUVECs. Atorvastatin (0.05, 0.1, 0.5, 1 µmol/L) could inhibit the production of IL18 in HUVECs activated by Ox-LDL (P < 0.05, P < 0.01), but not in 0.01 µmol/L (P < 0.05). The inhibitory effect was dose-dependent.

Conclusions atorvastatin can obviously inhibited the production of IL18 in HUVECs and enhanced the proliferation of HUVECs activated by Ox-LDL, which might improve the impaired endothelial cell beyond lipid regulation function.

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