Objectives To investigate the stimulative effect of D3 dopamine receptors on the insulin-like growth factors-1 (IGF-1)-induced vascular smooth muscle cells (VSMCs) migration and the mechanism underlying it.
Methods A10 cells were treated by IGF-1 with or without the presence of D3 receptor agonist, PD128907. Migration was determined by transwell assay. Protein of eNOS and p-eNOS expressions were determined by immunoblotting.
Results IGF-1 facilitated the migration of VSMCs in a concentration-dependent manner. The stimulatory effect of IGF-1 in VSMCs was reduced by the specific D3 receptor agonist, PD128907. D3 receptor antagonist, U99194, could block the anti-migration effect of PD128907. NOS and cGMP inhibitors, L-NAME and ODQ, suppressed the migration of A10 cells. PD128907 enhenced the expression of phosphorylation of eNOS, indicating that NO/cGMP was involved in the signalling pathway.
Conclusions These results suggested that activation of D3 dopamine receptors suppresses the IGF-1-mediated migration of VSMCs.