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GW24-e2368 Effect of dopamine D3 receptors on migration of vascular smooth muscle cell induced by insulin-like growth factor-1
  1. Kou Xun1,2,
  2. Chunyu Zeng1,2
  1. 1Department of Cardiology, Daping Hospital, The Third Military Medical University
  2. 2Chongqing Institute of Cardiology

Abstract

Objectives To investigate the stimulative effect of D3 dopamine receptors on the insulin-like growth factors-1 (IGF-1)-induced vascular smooth muscle cells (VSMCs) migration and the mechanism underlying it.

Methods A10 cells were treated by IGF-1 with or without the presence of D3 receptor agonist, PD128907. Migration was determined by transwell assay. Protein of eNOS and p-eNOS expressions were determined by immunoblotting.

Results IGF-1 facilitated the migration of VSMCs in a concentration-dependent manner. The stimulatory effect of IGF-1 in VSMCs was reduced by the specific D3 receptor agonist, PD128907. D3 receptor antagonist, U99194, could block the anti-migration effect of PD128907. NOS and cGMP inhibitors, L-NAME and ODQ, suppressed the migration of A10 cells. PD128907 enhenced the expression of phosphorylation of eNOS, indicating that NO/cGMP was involved in the signalling pathway.

Conclusions These results suggested that activation of D3 dopamine receptors suppresses the IGF-1-mediated migration of VSMCs.

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