Article Text

PDF
GW24-e2527 Effects of atorvastatin on BMP/Smad signaling in pulmonary arterial smooth muscle cells derived from pulmonary hypertensive rats
  1. Suli Zheng,
  2. Liangdi Xie,
  3. Zhi Jiang,
  4. Changsheng Xu
  1. Fujian Hypertension Research Institute, The First Affiliated Hospital of Fujian Medical University

Abstract

Objectives The present study was to investigate the effects of atorvastatin on BMP/Smad signalling in pulmonary arterial smooth muscle cells (PASMCs) derived from pulmonary artery hypertensive (PAH) rats.

Methods PAH was induced by a single intraperitoneal injection of MCT at a dose of 40mg/Kg. Mean pulmonary arterial pressure (MPAP) was measured by catheterisation; right ventricular hypertrophy index (RVHI) were calculated [RVHI = RV/(LV + S)]. Cultured PASMCs isolated from PAH rats were treated with atorvastatin in the concentrations ranging from 0- 10-5mol/l for 24h. Untreated PASMCs isolated from normal rats were used as control. The mRNA expressions of BMP2, BMPR2, Smad1 and Smad4 were determined by RT-PCR respectively, and the protein expressions of BMP2, BMPR2 and p-Smad1, Smad4 were determined by western-Blot.

Results MPAP(15.10 ± 0.72 vs 32.63 ± 0.98) and RVHI(24.94 ± 4.66 vs 52.60 ± 3.40) were higher in PAH rats, as compared with normal rats (P < 0.05 respectively). The mRNA expressions of BMP2, BMPR2,and Smad1, Smad4 in PASMCs derived from PAH rats were obviously lower than that from normal rats (P < 0.05). Atorvastatin induced an increase in mRNA expressions in the cultured PASMCs derived from PAH rats in a concentration-dependent manner (P < 0.05). The protein expressions of BMP2, BMPR2, Smad4 the and phosphorylation level of Smad1 in PASMCs derived from PAH rats were obviously lower than that from normal rats (P <0.05). Atorvastatin induced an increase in protein expressions and phosphorylation level in the cultured PASMCs derived from PAH rats (P <0.05).

Conclusions The expression of BMP/Smad signalling was abnormal in PASMCs derived from pulmonary artery hypertensive rats induced by MCT. Atorvastatin alleviated PAH may be related to the up-regulation of the expression of BMP/Smad signalling.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.