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GW24-e1352 Berberine mitigated cardiac hypoxiareoxygenation injury by Suppressed Autophagy and Reduced Cell Death via inhibition of the activation of AMPK-mTOR signalling pathway in rat H9c2 cells
  1. Huang Zhouqing,
  2. Ye Bozhi,
  3. Huang Weijian
  1. The First Affiliated Hospital of Wenzhou Medical College

Abstract

Objectives The present study was designed to investigate the effect of berberine on the autophagy level in rat H9c2 myocytes undergoing hypoxia/reoxygenation (H/R) injury and the mechanism involved in the process.

Methods H9c2 myocytes was cultured in DMEM without glucose and fetal bovine serum and subjected to H/R. The cell viability was assessed by MTT assay and the activity of autophagy was detected by electron microscopy and its component LC3-II. Western blotting was used to evaluate the expression level of AMPK, mTOR (Ser2481), SIRT1, BNIP3, and Beclin-1.

Results Autophagy level greatly upregulated in H9c2 myocytes during H/R, correlated with reduced cell survival. Berberine treatment significantly enhanced the H/R-induced cell viability and inhibited the cellular autophagy level through decreasing the expression of autophagy-related proteins such as SIRT1, BNIP3 and Beclin-1. In addition, we found that the phosphorylation of AMPK and the mammalian target of rapamycin (mTOR) at Ser2481 was increased during H/R in myocytes and both were attenuated by berberine.

Conclusions Berberine play its beneficial effects by decreasing the level of autophagy in H9c2 myocytes during H/R injury through suppressing the activation of AMPK-mTOR pathway and the expression of SIRT1, BNIP3 and Beclin-1.

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