Objectives To observe whether CRP influenced expressions of eNOS/NO in late endothelial outgrowth cells (EOCs) though signalling pathway mediated by receptor for advanced glycation end products (RAGE), and whether rosuvastatin could improve EOCs’ reendothelialisation influenced by CRP though restraining RAGE.
Methods The EOCs were treated with CRP of different concentrations and treating times, and EOCs were pre-incubated with rosuvastatin of different concentrations and then stimulated with 50ug/ml CRP. The eNOS of EPCs were measured by quantitative PCR and NO secretions were detected by NO nitrate reductive enzymatic. The RAGE protein expressions were checked by western blotting. MTT assay was used to test proliferation of EPCs, transwell assay for migration, and adhesiveness assay for adhesion.
Results The eNOS mRNA expression and NO secretions decreased significantly with CRP-stimulated concentration and time, and down-regulated by rosuvastatin. Also, the RAGE protein expression rised in a dose-dependent and time-dependent manner. The RAGE was significantly decreased by rosuvastatin with different dosages. After treatment with different dosage rosuvastatin, the vitro functions (migration and adhesion) of CRP-influenced EOCs were dosage-dependently upregulated. However, the proliferation had no changes.
Conclusions CRP could influence expressions of eNOS/NO though signalling pathway mediated by RAGE. Rosuvastatin could improve EOCs’ reendothelialisation influenced by CRP though restraining RAGE.