Article Text

PDF
GW24-e3651 Biological Effects of Ultrasound Mediated Nitric oxide Microbubbles Destruction on Rat Myocardium
  1. Shen Xiangbo1,
  2. Bian Yeping2,
  3. Ding Jiandong1,
  4. Chen Long1,
  5. Tong Jiayi1
  1. 1Institute of Cardiology, Southeast University
  2. 2Jiangsu Province Official Hospital, Nanjing

Abstract

Objectives: This study evaluates the biological effects of ultrasound combined with homemade nitric oxide (NO) microbubbles destruction on rat myocardium.

Methods Thirty-two rats were divided into four groups randomly. Ultrasound (1MHz, 1W/cm2) was applied to the anterior chest for ten minites in group A(US + NOMB), group B(US + MB) and group C(US + PBS), group D was set for contrast. Myocardium was harvested one week later, RT-PCR and western blotting were performed for testing stromal cell-derived factor-1(SDF-1) expression.

Results RT-PCR results showed that the level of SDF-1 was higher in group I (0.70 ± 0.018) than that in group II (0.65 ± 0.026), group III (0.23 ± 0.024) and group IV (0.02 ± 0.010), and all the P values were less than 0.05. Western blotting results showed that the level of SDF-1 was higher in group I (0.89 ± 0.024) than that in group II (0.78 ± 0.017), group III (0.49 ± 0.024) and group IV (0.22 ± 0.022), and all the P values were less than 0.05.

Conclusions No microbubble, as a novel microbubble, has a better effect in promoting SDF-1 expression, and can be used in stem cell transplantation.

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.