Objectives: This study evaluates the biological effects of ultrasound combined with homemade nitric oxide (NO) microbubbles destruction on rat myocardium.
Methods Thirty-two rats were divided into four groups randomly. Ultrasound (1MHz, 1W/cm2) was applied to the anterior chest for ten minites in group A(US + NOMB), group B(US + MB) and group C(US + PBS), group D was set for contrast. Myocardium was harvested one week later, RT-PCR and western blotting were performed for testing stromal cell-derived factor-1(SDF-1) expression.
Results RT-PCR results showed that the level of SDF-1 was higher in group I (0.70 ± 0.018) than that in group II (0.65 ± 0.026), group III (0.23 ± 0.024) and group IV (0.02 ± 0.010), and all the P values were less than 0.05. Western blotting results showed that the level of SDF-1 was higher in group I (0.89 ± 0.024) than that in group II (0.78 ± 0.017), group III (0.49 ± 0.024) and group IV (0.22 ± 0.022), and all the P values were less than 0.05.
Conclusions No microbubble, as a novel microbubble, has a better effect in promoting SDF-1 expression, and can be used in stem cell transplantation.