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Increased serum levels and expression of S100A8/A9 complex in infiltrated neutrophils in atherosclerotic plaque of unstable angina
  1. Shoichi Miyamoto
  1. Division of Cardiology, Kitano Hospital, Tadukekofukai Medical Research Institute, Osaka, Japan
    1. Makiko Ueda
    1. Department of Pathology, Osaka City University Graduate School of Medicine, Osaka, Japan
      1. Masaki Ikemoto
      1. Human Health Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan
        1. Takahiko Naruko
        1. Department of Cardiology, Osaka City General Hospital, Osaka, Japan
          1. Akira Itoh
          1. Department of Cardiology, Osaka City General Hospital, Osaka, Japan
            1. Shun-ichi Tamaki
            1. Division of Cardiology, Takeda Hospital, Kyoto, Japan
              1. Ryuji Nohara
              1. Division of Cardiology, Kitano Hospital, Tadukekofukai Medical Research Institute, Osaka, Japan
                1. Fumio Terasaki
                1. Third Department of Internal Medicine, Osaka Medical College, Takatsuki, Japan
                  1. Shigetake Sasayama
                  1. Heart Bio-Mechanics Center, Doshisha University, Kyoto, Japan
                    1. Masatoshi Fujita (mfujita{at}kuhp.kyoto-u.ac.jp)
                    1. Human Health Sciences, Kyoto University Graduate School of Medicine, Kyoto, Japan

                      Abstract

                      Objective The S100A8/A9 complex is expressed in a subset of activated neutrophils and macrophages in acute inflammatory lesions associated with various diseases. The purpose of this study was to investigate; (1) whether serum S100A8/A9 levels are increased in patients with unstable angina (UA), and (2) whether S100A8/A9 expression is upregulated in coronary atherosclerotic plaques of patients with UA.

                      Design Serum S100A8/A9 levels in 39 patients with stable angina (SA) and 53 patients with UA were measured. In addition, we immunohistochemically studied the presence of the S100A8/A9 complex in directional coronary atherectomy specimens. For the identification of the cell types which stain positive for S100A8/A9, immunodouble staining with neutrophils and macrophages was carried out.

                      Results Serum S100A8/A9 levels were significantly higher in patients with UA than in those with SA (3.25 ± 3.08 µg/ml versus 0.77 ± 0.31 µg/ml, p < 0.05). In patients with UA, the immunodouble staining clearly revealed that the S100A8/A9 complex was expressed in infiltrated neutrophils and occasional macrophages. The S100A8/A9-positive area was significantly higher (18.3 ± 14.2 % versus 1.3 ± 2.4 %, p < 0.0001) in UA than in SA.

                      Conclusions The S100A8/A9 complex may be involved in the inflammatory process of coronary atherosclerotic plaques in patients with UA.

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