Objective : Peripheral blood stem cell (PBSC) is one of the most promising stem cell sources for treatment of ischemic heart disease. However, the experience of mobilization and collection of PBSC using granulocyte colony-stimulating factor (G-CSF) in patients with myocardial infarction (MI) is still limited. We report our experiences about the feasibility and safety of collection of mobilized PBSC with G-CSF in MI patients, and the influence of acute ischemia on efficacy of PBSC collection.
Methods: Total 74 patients with acute or old myocardial infarction (AMI vs. OMI, n=46 and 28) underwent PBSC collection after administration of G-CSF with dose of 5μg/kg for 3days. Flow cytometric analysis of cell surface marker was performed.
Results: No evidence of inflammation or ischemia was observed during G-CSF mobilization and peripheral blood stem cell collection. The yield of CD34+ cells were 12.9 ±15.92 x103 /μL (5.04±5.30% of total cells) with product volume of 37.9±8.4 mL after 5,650±987 mL of blood were processed during PBSC collection. Stem cell mobilization and collection by G-CSF is more efficient in AMI than in OMI, and proportions of cells positive for VE-cadherin or KDR/CD34 are significantly greater in AMI than in OMI (p<0.01).
Conclusion: We could obtain sufficient numbers of PBSC for intracoronary infusion by G-CSF based mobilization strategy without complications even in patients with myocardial infarction. PBSC collection after mobilization with G-CSF was a safe and feasible method of stem cell collection for therapeutic purpose in patients with myocardial infarction.
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