Construction of a human cardiovascular cDNA microarray: portrait of the failing heart

Biochem Biophys Res Commun. 2001 Feb 2;280(4):964-9. doi: 10.1006/bbrc.2000.4137.

Abstract

Identifying key genes that regulate the complex diseases of the cardiovascular system can be greatly facilitated with the use of microarrays. In an effort to obtain a global portrait of gene expression in the failing heart, we have constructed in-house a glass microscope slide cDNA microarray (termed "CardioChip") containing 10,368 redundant and randomly-selected sequenced expressed sequence tags (representing known genes, other matched ESTs, and novel, unmatched ESTs) derived from several human heart and artery cDNA libraries. From our preliminary data with Cy3- and Cy5-labeled probes, we have identified 38 transcripts showing a minimum twofold differential expression, among which are several novel or previously-uncharacterized genes. This array-representing what we believe to be the largest cardiovascular-based cDNA array to date-establishes a practical and invaluable platform for obtaining a global genetic portrait of complex cardiovascular diseases, particularly in the failing heart.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arteries / metabolism
  • Cardiovascular System / metabolism
  • Cardiovascular System / physiopathology*
  • DNA, Complementary / metabolism
  • Expressed Sequence Tags
  • Gene Expression
  • Heart / physiopathology*
  • Humans
  • Image Processing, Computer-Assisted
  • Myocardium / metabolism
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Messenger / metabolism

Substances

  • DNA, Complementary
  • RNA, Messenger