Primer used to detect mRNA expression by reverse transcriptase polymerase chain reaction
| Forward | Reverse | PCR product | TA | Accession code | |
|---|---|---|---|---|---|
| MMP, matrix metalloproteinase; PCR, polymerase chain reaction; TA, annealing temperature; TIMP, tissue inhibitor of metalloproteinases. | |||||
| MMP-1 | AgCAAACACATCTgACCTAC | TAAAgAACATCACTTCTCCC | 564 | 55.3 | X 54925 |
| MMP-2 | AgTCTgCTCTgCCTATCCTC | ACACCCATATCTgTCTTCCC | 833 | 59.8 | NM00453 |
| MMP-3 | ACCCACTCTATCACTCACTCAC | CTgTTACTCTTCAAAgTgTgTgTC | 536 | 56.9 | J 03209 |
| MMP-9 | CCAgTTTCCATTCATCTTCC | ACAgTAgTggCCgTAgAAgg | 479 | 55.0 | NM004994 |
| CCCTTgTgCTCTTCCCTg (nested) | CCCgTCCTTCCCgTCgAAg (nested) | 303 | 60.0 | NM004994 | |
| TIMP-2 | gAAACgACATTTATggCAAC | gATgTTCTTCTCTgTgACCC | 377 | 58.3 | J 05593 |