MMP concentration (ELISA) | Zymographic activity of MMP | mRNA expression (RT-PCR) (MMP/ GAPDH in AU) | |||||||
---|---|---|---|---|---|---|---|---|---|
MMP-2 (ng/ml) | MMP-9 (ng/ml) | MMP-2 (AU) | MMP-9 (AU) | MMP-1/ GAPDH | MMP-2/ GAPDH | MMP-3/ GAPDH | MMP-9/ GAPDH | TIMP-2/ GAPDH | |
Values are mean (SD); mRNA expression is given in arbitrary units expressing the videodensitometrically determined ratio of MMP to GAPDH RT-PCR. | |||||||||
*p<0.05 v control. | |||||||||
†Nested reverse transcriptase polymerase chain reaction (RT-PCR). | |||||||||
AU, arbitrary units; CAD, coronary artery disease; ELISA, enzyme linked immunosorbent assay; GAPDH, glyceraldehyde-3-phosphodehydrogenase; IDCM, idiopathic dilated cardiomyopathy; MMP, matrix matalloproteinase; TIMP, tissue inhibitor of matrix metalloproteinases. | |||||||||
IDCM (n=16) | 3.68 (0.41)* | 2.11 (0.54)* | 1196 (74)* | 802 (132)* | 0 | 0.492 | 0 | 0.22 | 1.16 |
CAD (n=13) | 0.7 (0.26) | 1.6 (0.35)* | 571 (71) | 549 (71)* | 0 | 0.329 | 0 | <0.005† | 0.6 |
Control (n=6) | 0.89 (0.35) | 0.53 (0.15) | 989 (158) | 245 (58) | 0.012 | 0.7 | 0 | 0.033 | 1.06 |