In vitro assays of rabbit aortic smooth muscle cell proliferation and viability
| Drug | Assay | No. | Concentration | |||||
|---|---|---|---|---|---|---|---|---|
| Control | 1% Ethanol | 10−8 mol/l | 10−7 mol/l | 10−6 mol/l | 10−5 mol/l | |||
| Data are mean (SD). | ||||||||
| Cells are the number of smooth muscle cells in culture wells as quantified by Coulter Counter; protein is the amount of total protein in culture wells as quantified by BCA protein assay kit; viability was determined by neutral red staining before total protein quantification; relative viability is the ratio of neutral red staining on total protein quantification. All concentrations are a percentage of the control value. | ||||||||
| *p<0.05; **p<0.01 versus control by one way analysis of variance followed by Dunnett’s post hoc test. | ||||||||
| NA, not applicable, as methotrexate was dissolved in NaCl. | ||||||||
| Methotrexate | Cells | 4 | 100 | NA | 108 (24) | 107 (23) | 94 (18) | 100 (14) |
| Protein | 3 | 100 | NA | 97 (3) | 97 (6) | 95 (8) | 97 (9) | |
| Viability | 3 | 100 | NA | 99 (6) | 99 (4) | 99 (4) | 98 (5) | |
| Relative viability | 3 | 100 | NA | 102 (6) | 101 (3) | 104 (10) | 102 (5) | |
| Paclitaxel | Cells | 4 | 100 | 100 (8) | 55 (13)** | 22 (7)** | 13 (3)** | 5 (2)** |
| Protein | 3 | 100 | 97 (4) | 95 (4) | 79 (3)** | 42 (2)** | 12 (9)** | |
| Viability | 3 | 100 | 90 (3)** | 88 (1)** | 66 (4)** | 32 (2)** | 5 (1)** | |
| Relative viability | 3 | 100 | 94 (5) | 94 (4) | 83 (7) | 76 (1)* | 46 (18)** | |