Primer sequences and reaction conditions used for amplification of viral and myoglobin sequences (Y = C + T)
| Sequence amplified | Primer sequence | Annealing temp | MgCl2(mM) | No of cycles | Amplicon (bp) | |
| Enterovirus 5′ UTR | First round PCR | 50°C | 2.0 | 30 | 228 | |
| Nucleotide positions 417–645 | ||||||
| 5′-GGTGYGAAGAGYCTAYTGAG | ||||||
| 5′-CACYGGATGGCYATTCCA | ||||||
| Nested reaction | 52°C | 1.8 | 25 | 146 | ||
| Nucleotide positions 456–602 | ||||||
| 5′-CCCCTGAATGCGGCTAAT | ||||||
| 5′-ATTGTCACCATAAGCAGCCA | ||||||
| HCMV UL83 region | First round PCR | 57°C | 1.5 | 40 | 449 | |
| Nucleotide positions 120032–120489 | ||||||
| 5′-TCTCGCGTATGGCTTG | ||||||
| 5′-GTCAGCGTTCGTGTTTCC | ||||||
| Nested reaction | 55°C | 1.5 | 30 | 233 | ||
| Nucleotide positions 120144–120377 | ||||||
| 5′-TGAGGTAAAAGCCACATCCAG | ||||||
| 5′-GACCAGTACGTCAAGGTGTAC | ||||||
| Adenovirus hexon region | First round PCR | 60°C | 2.0 | 40 | 300 | |
| Nucleotide positions 18858–19158 | ||||||
| 5′-GCCGCAGTGGTCTTACATGCACATC | ||||||
| 5′-CAGCACGCCGCGGATGTCAAAGT | ||||||
| Nested reaction | 60°C | 2.0 | 30 | 142 | ||
| Nucleotide positions 18937–19079 | ||||||
| 5′-GCCACCGAGACGTACTTCAGCCTG | ||||||
| 5′-TTGTACGAGTACGCGGTATCCTCGCGGTC | ||||||
| Myoglobin exons 1 and 3 | Amplification from DNA | 55°C | 1.5 | 35 | 439 | |
| 5′-AACATGACAGGTCCTCTTGG | ||||||
| 5′-CATGGCGCAGTCTGAA | ||||||
| Amplification from cDNA | 55°C | 1.5 | 40 | 299 | ||
| 5′TTGGCAGGAAGTCCTCATCA | ||||||
| 5′-AGCTCCAGGGCCTTGTTCAT | ||||||
bp, base pair; HCMV UL83, human cytomegalovirus unique long; PCR, polymerase chain reaction.