Elsevier

Microbial Pathogenesis

Volume 24, Issue 2, February 1998, Pages 101-109
Microbial Pathogenesis

Regular Article
Lipopolysaccharide induces apoptosis in a bovine endothelial cell line via a soluble CD14 dependent pathway

https://doi.org/10.1006/mpat.1997.0178Get rights and content

Abstract

We investigated the mode of bovine endothelial cell death induced by lipopolysaccharide (LPS). Inter-nucleosomal DNA fragmentation was observed indicating apoptotic cell death. Apoptosis was confirmed by examining cell morphology using confocal laser scanning and transmission electron microscopy, which revealed characteristic nuclear condensation in LPS-treated endothelial cells. Apoptosis was blocked by a monoclonal antibody against CD14, suggesting that LPS triggers apoptosis via a soluble CD14 (sCD14) dependent mechanism.

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