Abstract
A new commercial test for chlamydial serology, the MRL-Micro-Immunofluorescent Test (MRL; MRL Diagnostics, USA) was compared with the standard microimmunofluorescence test (MIF) using sera from 246 patients.Chlamydia pneumoniae immunoglobulin G (IgG) antibodies were detected in 46.3% (MIF) and 64.2% (MRL) of sera andChlamydia trachomatis IgG in 23.2% (MIF) and 25.2% (MRL);Chlamydia psittaci IgG antibodies were found with the MRL in 1 % of the sera from a general population and in 17.3% of preselected sera with elevated complement fixation titers. Titers were usually higher with the MRL. IgG titers of ≥ 1∶512 were detected in only 2% of sera using the standard MIF but in 30% using the MRL. In 16 sera from threeChlamydia pneumoniae culturepositive patients, the diagnosis of acute infection could be confirmed serologically in one with the MRL test but in none with the MIF test, indicating a higher sensitivity of the MRL.
Similar content being viewed by others
References
Kuo CC, Jackson LA, Campbell LA, Grayston JT:Chlamydia pneumoniae (TWAR). Clinical Microbiology Reviews 1995, 8: 451–461.
Centers for Disease Control: Recommendations for the prevention and management ofChlamydia trachomatis infections, 1993. Morbidity and Mortality Weekly Report 1993: 42, 1–39.
Black CM: Current methods of laboratory diagnosis ofChlamydia trachomatis infections. Clinical Microbiology Reviews 1997, 10: 160–184.
Schachter J: Chlamydiae. In: Rose NR, de Macario EC, Fahey JL, Friedman H, Renn GM (ed): Manual of clinical laboratory immunology. American Society for Microbiology, Washington DC, 1992, p. 661–666.
Ekman MR, Leinonen M, Syrjälä H, Linnanmäki E, Kujala P, Saikku P: Evaluation of serological methods in the diagnosis ofChlamydia pneumoniae pneumonia during an epidemic in Finland. European Journal of Clinical Microbiology & Infectious Diseases 1993, 12: 756–760.
Wang SP, Grayston JT: Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. American Journal of Ophthalmology 1970, 70: 367–374.
Bergström K, Domeika M, Vaitkiene D, Persson K, Mardh PA: Prevalence ofChlamydia trachomatis, Chlamydia psittaci andChlamydia pneumoniae antibodies in blood donors and attendees of STD clinics. Clinical Microbiology and Infection 1996, 1: 253–260.
Freidank HM, Brauer D: Prevalence of antibodies toChlamydia pneumoniae TWAR in a group of German medical students. Journal of Infection 1993, 27: 89–93.
Grayston JT: Infections caused byChlamydia pneumoniae strain TWAR. Clinical Infectious Diseases 1992, 15: 757–763.
Steinhoff D, Lode H, Ruckdeschel G, Heidrich F, Rolfs A, Fehrenbach FJ, Mauch H, Höffken G, Wagner J:Chlamydia pneumoniae as a cause of community-acquired pneumonia in hospitalized patients in Berlin. Clinical Infectious Diseases 1996, 22: 958–964.
Wang SP, Grayston JT: Microimmunofluorescence serological studies with the TWAR organism. In: Oriel D, Ridgway G, Schachter J, Taylor-Robinson D, Ward M (ed): Chlamydial infections — 1986. Cambridge University Press, Cambridge, 1986, p. 329–332.
Wang SP, Grayston JT: The similarity ofChlamydia pneumoniae (TWAR) isolates as antigen in the microimmunofluorescence (MIF) test. In: Orfila J, Byrne Gl, Cherneskey MA, Grayston JT, Jones RP, Ridgway GL, Saikku P, Schachter J, Stamm WE, Stepens RS (ed): Chlamydial infections-1994. Societa Editrice Esculapio, Bologna, Italy, 1994, p. 181–184.
Kern DG, Neill MA, Schachter J: A seroepidemiologic study ofChlamydia pneumoniae in Rhode Island. Chest 1993, 104: 208–213.
Chirgwin K, Roblin PM, Gelling M, Hammerschlag MR, Schachter J: Infection withChlamydia pneumoniae in Brooklyn. Journal of Infectious Diseases 1991, 163: 757–761.
Gaydos CA, Roblin PM, Hammerschlag MR; Hyman CL, Eiden JJ, Schachter J, Quinn TC: Diagnostic utility of PCR-enzyme immunoassay, culture, and serology for detection ofChlamydia pneumoniae in symptomatic and asymptomatic patients. Journal of Clinical Microbiology 1994, 32: 903–905.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Freidank, H.M., Vögele, H. & Eckert, K. Evaluation of a new commercial microimmunofluorescence test for detection of antibodies toChlamydia pneumoniae, Chlamydia trachomatis, andChlamydia psittaci . Eur. J. Clin. Microbiol. Infect. Dis. 16, 685–688 (1997). https://doi.org/10.1007/BF01708561
Issue Date:
DOI: https://doi.org/10.1007/BF01708561