Summary
Whereas tissue factor (TF), a 47 kDa transmembrane glycoprotein, is constitutively present in certain tissues such as epithelial tissue, brain, and placenta, it is normally not expressed by cells within the vasculature. However, inflammatory mediators including bacterial lipopolysaccharide (LPS) can stimulate the expression of cell surface procoagulant activity (PCA) on monocytes. In our present study the kinetics (over 24 h) of molecular TF expression on LPS-stimulated monocytes analyzed by flow cytometry corresponds closely to functional PCA of human mononuclear blood cells (MBC). Both PCA and TF expression on monocytes were rapid events reaching their maximum after about 6 h of stimulation. At this time approximately 70–80% of monocytes had also achieved maximum anti-TF MAb receptor density. For certain analytical applications, monitoring of molecular TF expression on monocytes by flow cytometry using anti-TF MAb is favorable because there is no influence by PCA inhibitors.
This is a preview of subscription content, log in via an institution to check access.
References
Altieri DC, Edgington TS (1988) Sequential receptor cascade for coagulation proteins on monocytes. J Biol Chem 264: 2969–2972
Carlsen E, Gaudernack G, Fillon-Myklebust C, Pettersen KS, Prydz H (1989) Allogeneic induction of thromboplastin synthesis in monocytes and endothelial cells. Biphasic effect of cyclosporin A. Clin Exp Immunol 76: 428–433
Drake TA, Morrissey JH, Edgington TS (1989) Selective cellular expression of tissue factor in human tissues. Am J Pathol 134: 1087–1097
Geczy CL, Hopper KE (1981) A mechanism of migration inhibition in delayed-type hypersensitivity reactions. II Lymphokines promote procoagulant activity of macrophages in vitro. J Immunol 126: 1059–1064
Gregory SA, Edgington TS (1985) Tissue factor induction in human monocytes. J Clin Invest 76: 2440–2445
Helin HJ, Fox RI, Edgington TS (1983) The instructor cell for the human procoagulant monocyte response to bacterial lipopolysaccharide is a Leu-3a+ T cell by fluorescence-activated cell sorting. J Immunol 131: 749–752
Henry MM, Moore JN (1988) Endotoxin-induced procoagulant activity in equine peripheral blood monocytes. Circ Shock 26: 297–309
Lal RB, Edison LJ, Chused TM (1988) Fixation and long-term storage of human lymphocytes for surface marker analysis by flow cytometry. Cytometry 9: 213–219
Levy GA, Schwartz BS, Edgington TS (1981) The kinetics and metabolic requirements for direct lymphocyte induction of human procoagulant monokines by bacterial lipopolysaccharide. J Immunol 127: 357–362
Lorenzet R, Peri G, Locati D, Allavena P, Colucci M, Semeraro N, Mantovani A, Donati MB (1983) Generation of procoagulant activity by mononuclear phagocytes: a possible mechanism contributing to blood clotting activation within malignant tissues. Blood 62: 271–273
Mackman N, Morrissey JH, Fowler B, Edgington TS (1989) Complete sequence of the human tissue factor gene, a highly regulated cellular receptor that initiates the coagulation protease cascade. Biochemistry 28: 1755–1762
Miller CL, Graziano C, Lim RC, Chin M (1981) Generation of tissue factor by patient monocytes: Correlation to thromboembolic complications. Thromb Haemost 46: 489–495
Morrissey JH, Fair DS, Edgington TS (1987) Monoclonal antibody analysis of purified and cell-associated tissue factor. Thromb Res 52: 247–261
Morrissey JH, Fakhrai H, Edgington TS (1987) Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell 50: 129–135
Müller M, Flössel C (1988) Induktion und Bestimmung prokoagulatorischer Aktivität mononukleärer Zellen des menschlichen Blutes. Z Med Lab Diagn 29: 363–371
Rana SV, Reimers HJ, Pathikonda MS, Bajaj SP (1988) Expression of tissue factor and factor VIIa/tissue factor inhibitor activity in endotoxin or phorbol ester stimulated U 937 monocyte-like cells. Blood 71: 259–262
Ryan J, Geczy CL (1988) Macrophage procoagulant-inducing factor. J Immunol 141: 2110–2117
Schwartz BS, Levy GA, Edgington TS (1982) Immune complex-induced human monocyte procoagulant activity. II Cellular kinetics and metabolic requirements. J Immunol 128: 1037–1042
Surprenant YM, Zuckerman SH (1989) A novel microtiter plate assay for the quantitation of procoagulant activity on adherent monocytes, macrophage and endothelial cells. Thromb Res 53: 339–346
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Luther, T., Flössel, C., Hietschhold, V. et al. Flow cytometric analysis of tissue factor (TF) expression on stimulated monocytes — comparison to procoagulant activity of mononuclear blood cells. Blut 61, 375–378 (1990). https://doi.org/10.1007/BF01738553
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01738553