Abstract
We observed that normal plasma dramatically reduces neutrophil-endothelial adhesion. Therefore, we identified factors in plasma which might limit PMN adhesion in vitro. We found that the anti-adhesive effect was not mediated by vasoactive lipids present in plasma. Immunoprecipitation of soluble adhesion molecules, P and E-selectins and ICAM-1 restored PMN adhesion to control values. We further examined whether soluble adhesion molecules in plasma might also regulate PMN endothelial migration in response to fMLP (10−6 M). Plasma significantly reduced PMN migration, and this effect was prevented only by the simultaneous removal of soluble P and E selectins and ICAM-1 together, but not individually. These data show that soluble selectins and ICAM-1 may regulate PMN adhesion and diapedesis, and that alterations in the levels of these molecules may regulate PMN-endothelial interactions in vivo.
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Ohno, N., Ichikawa, H., Coe, L. et al. Soluble Selectins and ICAM-1 Modulate Neutrophil-Endothelial Adhesion and Diapedesis in Vitro. Inflammation 21, 313–324 (1997). https://doi.org/10.1023/A:1027349900279
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DOI: https://doi.org/10.1023/A:1027349900279