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Soluble Selectins and ICAM-1 Modulate Neutrophil-Endothelial Adhesion and Diapedesis in Vitro

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We observed that normal plasma dramatically reduces neutrophil-endothelial adhesion. Therefore, we identified factors in plasma which might limit PMN adhesion in vitro. We found that the anti-adhesive effect was not mediated by vasoactive lipids present in plasma. Immunoprecipitation of soluble adhesion molecules, P and E-selectins and ICAM-1 restored PMN adhesion to control values. We further examined whether soluble adhesion molecules in plasma might also regulate PMN endothelial migration in response to fMLP (10−6 M). Plasma significantly reduced PMN migration, and this effect was prevented only by the simultaneous removal of soluble P and E selectins and ICAM-1 together, but not individually. These data show that soluble selectins and ICAM-1 may regulate PMN adhesion and diapedesis, and that alterations in the levels of these molecules may regulate PMN-endothelial interactions in vivo.

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REFERENCES

  1. ADKINS, W. K., and A. E. TAYLOR, 1990. Role of xanthine oxidase and neutrophils in ischemia-reperfusion injury in rabbit lung. J. Appl. Physiol. 69:2012–2018.

    Google Scholar 

  2. ARFORS, K. E., C. LUNDBERG, L. LINDBOM, K. LUNDBERG, P. G. BEATTY, and J. M. HARLAN. 1987. A monoclonal antibody to the membrane glycoprotein complex CD 1 8 inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo. Blood 69:338–340.

    Google Scholar 

  3. ASHBAUGH, D. G., D. B. BIGELOW, T. L. PETTY, and B. E. LEVINE. 1967. Acute respiratory distress in adults. Lancet 2:319–323.

    Google Scholar 

  4. BANKS, R. E., A. J. H. GEARING, I. K. HEMINGWAY, D. R. NORFOLK, T. J. PERREN, and P. J. SELBY. 1993. Circulating intercellular adhesion molecule-1 (ICAM-1), E-selectin and vascular cell adhesion molecules (VCAM-1) in human malignancies. Br. J. Cancer 68:122–134.

    Google Scholar 

  5. BERGER, N. A. 1991. Oxidant-induced cytotoxicity: A challenge for metabolic modulation. Am. J. Respir. Cell Mol. Biol. 4:1–3.

    Google Scholar 

  6. BRIGHAM, K. L., R. E. BOWERS, and J. HAYNES. 1979. Increased sheep lung vascular permeability caused by E. coli endotoxin. Circ. Res. 45:292–297.

    Google Scholar 

  7. COWLEY, H. C., D. HENEY, A. J. GEARING, I. HEMINGWAY, and N. R. WEBSTER. 1994. Increased circulating adhesion molecule concentrations in patients with the systemic inflammatory response syndrome: A prospective cohort study. Crit. Care Med. 22(4):651–657.

    Google Scholar 

  8. CUSH, J. J., R. ROTHLEIN, H. B. LINDSLEY, E. A. MAINOLFI, and P. E. LIPSKY. Increased levels of circulating intercellular adhesion molecule I in the sera of patients with rheumatoid arthritis. Arthritis and Rheumatism, 36:1098–1102.

  9. DEMLING, R. H. 1990. Current concepts on the Adult Respiratory Distress Syndrome. Circulatory Shock, 30:297–309.

    Google Scholar 

  10. DUNLOP, L. C., M. P. SKINNER, L. J. BENDALL, E. J. FAVALORO, P. A. CASTALDI, J. J. GONNAN, J. R. GAMBLE, M. A. VADAS, and M. C. BERNDT, 1992. Characterization of GNT-140 (P-Selectin) as a circulating plasma protein. J. Exp. Med. 175:1147–1150.

    Google Scholar 

  11. EICHHOLTZ, T., K. JALINK, I. FAHRENFORT, and W. H. MOOLENAAR. 1993. The bioactive phospholipid lysophosphatidic acid is released from activated platelets. Biochem. J. 291:677–680.

    Google Scholar 

  12. ERLANSSON, M., D. BERGQVIST, N. H. PERSSON, and E. SVENSJO, 1991. Modification of postischemic increase of leukocyte adhesion and vascular permeability in the hamster by iloprost. Prostaglandins 41:157–168.

    Google Scholar 

  13. GRANGER, D. N., J. N. BENOIT, M. SUZUKI, and M. B. GRISHMA. 1989. Leukocyte adherence to venular endothelium during ischemia-reperfusion. Am. J. Physiol. 257:G683–688.

    Google Scholar 

  14. GRANGER, D. N., I. KUROSE, and P. R. KVIETYS. 1995. Modulation of leukocyte adherence and emigration during ischemia and reperfusion. In ‘Physiology and Pathophysiology of Leukocyte Adhesion’ Eds. D. N. Granger and G. W. Schmidt-Schonbiem, Oxford Press, pp. 323–339.

  15. HALLMAN, R., M. A. JUTILA, C. W. SMITH, D. C. ANDERSON, T. K. KISHIMOTO, and E. C. BUCHER. 1991. The peripheral lymph homing receptor, LECAM-1, is involved in CD18-independent adhesion of human neutrophils to endothelium. Biochem. Biophys. Res. Commun. 174:236–243.

    Google Scholar 

  16. HARLAN, J. M., B. R. SCHWARTZ, M. A. REIDY, S. M. SCHWARTZ, H. OCHS, L. A. HARKER. 1985. Activated neutrophils disrupt endothelial monolayer integrity by an oxygen radical independent mechanism, neutrophils increase permeability. Lab Invest. 52:141–150.

    Google Scholar 

  17. HASELTON, F. R., J. S. ALEXANDER, S. N. MUELLER, and A. P. FISHMAN. Modulation of endothelial paracellular permeability: A mechanistic approach, In: Endothelial cell biology in health and disease. Eds. N. Simionscu and M. Simionscu Plenum Press, New York. pp. 103–126.

  18. INAUEN, W., D. N. GRANGER, C. J. MEININGER, M. E. SCHELLING, H. J. GRANGER, and P. R. KVIETYS. An in vitro model of ???? reperfusion-induced microvascular injury. Am. J. Physiol. 1990. 259: Gastrointest. Liver Physiol. 22): G134–G139.

    Google Scholar 

  19. INAUEN, W., D. N. GRANGER, C. J. MEININGER, M. E. SCHELLING, H. J. GRANGER, and P. R. KVIETYS. 1990. Anoxia reoxygenation-induced, neutrophil-mediated endothelial cell injury: role of elastase. Am. J. Physiol. 259: (Heart Circ. Physiol. 28) H925–H931.

    Google Scholar 

  20. ISMAIL, G., M. L. MORGANROTH, R. F. TODD III, and L. A. BOXER. 1987. Prevention of pulmonary injury in isolated perfused rat lungs by activated human neutrophils preincubated with anti-Mo I monoclonal antibody. Blood 69:1167–1174.

    Google Scholar 

  21. JACOBSON, P. B. and D. J. SCHRIER. 1993. Regulation of CDI lb/CD18 expression in human neutrophils by phospholipase A2. J. Immunol. 151:5639–5652.

    Google Scholar 

  22. KASLOVSKY, R. A., M. J. HORGAN, H. LUM, B. K. MC CANDLES, N. GILBOA, S. D. WRIGHT, and A. B. MALIK. 1990. Pulmonary edema produced by phagocytosing neutrophils: protective effect of monoclonal antibody against phagocyte CD-18 integrin. Circ. Res. 67:795–802.

    Google Scholar 

  23. KATAYAMA, M., M. HANDA, Y. ARAKI, H. AMBO, Y. KAWAI, K. WATANABE, and Y. IKEDA. Soluble P-selectin is present in normal circulation and its plasma level is elevated in patients with thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome. Br. J. Haematology 84:702–710.

  24. KIFLACKEY, J. J. F. and B. A. KILLACKEY. 1990. Neutrophil-mediated increased permeability of microcarrier cultured endothelial monolayers: a model for the in vitro study of neutrophil-dependent mediators of vasopermeability. Can. J. Physiol. Pharmacol. 68:836–844.

    Google Scholar 

  25. KVIETYS, P. R., W. INAUEN, B. R. BACON, and M. B. GRISHAM. 1989. Xanthine oxidase-induced injury to endothelium: role of intracellular iron and hydroxyl radical. Am. J. Physiol. 257 (Heart Circ. Physiol. 26): H1640–1646.

    Google Scholar 

  26. LEIRISALO-REPO, M. 1994. The present knowledge of the inflammatory process and the inflammatory mediators. Pharmacol. Toxicol. 75,suppl. 2: 1–3.

    Google Scholar 

  27. LUSCINSKAS, F. W., M. I. CYBULSKY, J. KIELY, C. S. PECKINS, V. M. DAVIS, and M. A. GIMBRONE. Cytokine-activated human endothelial monolayers support enhanced neutrophil transmigration via a mechanism involving both endothelial-leukocyte I adhesion molecule and intercellular adhesion molecule 1. J. Immunol. 146:1617–1625.

  28. NEWMAN, W., L. D. BEALL, C. W. CARSON, G. G. HUNDER, N. GRABEN, Z. X. RANDHAWA, T. V. GOPAL, J. WIENER-KRONISH, and M. A. MATTHAY. 1993. Soluble E-selectin is found in supernatants of activated endothelial cells and is elevated in serum of patients with septic shock. J. Immunol. 150:644–654.

    Google Scholar 

  29. NEWMAN, W., L. D. BEALL, C. W. CARSON, G. G. HUNDER, N. GRABEN, Z. L. RANDHAWA, T. V. GOPAL, J. WIENER-KRONISH, and M. A. MATHAY. 1993. Soluble E-selectin is found in supernatants of activated endothelial cells and is elevated in the serum of patients with septic shock. J. Immunol. 150:644–654.

    Google Scholar 

  30. ROTHLEIN, R., E. A. MAINOLFI, M. CZAJKOWSKI, and S. D. MARLIN. 1991. A form of circulating ICAM-1 in human serum. J. Immunol. 147:3788–3793.

    Google Scholar 

  31. SHAPPELL, S. B., C. TOMAN, D. C. ANDERSON, A. A. TAYLOR, M. L. ENTMAN, and C. SMITH. 1990. W. Mac-I (CD II/CD 1 8) mediates adherence-dependent hydrogen peroxide production by human and canine neutrophils. J. Immunol. 144:2702–2711.

    Google Scholar 

  32. SHASBY, D. M., S. E. LIND, S. S. SHASBY, J. C. GOLDSMEITH, and G. W. HUNNINGHAKE, 1985. Reversible oxidant induced increases in albumin transfer across cultured endothelium-Alterations in cell shape and calcium homeostasis. Blood 65:605–614.

    Google Scholar 

  33. SHASBY, D. M., S. S. SHASBY, M. J. PEACH. 1983. Granulocytes and phorbol myristate acetate increase permeability to albumfin of cultured endothelial monolayers and isolated perfused lungs. Am. Rev. Respir. Dis. 127:143.

    Google Scholar 

  34. SHASBY, D. M., K. M. VAN BENTHUYSEN, R. M. TATE, S. S. SHASBY, I. MC MURTY, and J. E. REPINE, 1982. Granulocytes mediate acute edematous lung injury in rabbits and in isolated rabbit lungs perfused with phorbol myristate acetate: Role of oxygen radicals. Am. Rev. Respir. Dis. 124:433.

    Google Scholar 

  35. SHEPRO, D., M. ROSENTHAL, L. BATBOUTA, and F. BELAMARICH. 1974. The cultivation of aortic endothelium. Anat. Rec. 78:523.

    Google Scholar 

  36. SPERTINI, O., F. W. LUSCINSKAS, G. S. KANSAS, J. M. MUNRO, J. D. CNIFFIN, M. A. GIMBRONE, JR., and T. F. TEDDER. 1991. Leukocyte adhesion molecule-I (LAM-1, L-selectin) interacts with an inducible endothelial cell ligang to support leukocyte adhesion. J. Immunol. 147:2565–2573.

    Google Scholar 

  37. VOELKEL, N. F. 1989. The Adult Respiratory Distress Syndrome. Klin. Wochenschr. 67:559–567.

    Google Scholar 

  38. WEISS, S. J. 1989. Tissue destruction by neutrophils. New Engl. J. of Med. 320:365–376.

    Google Scholar 

  39. WORTHEN, S. G., N. AVDI, S. VUKAJIOVICH and P. S. TOBIAS. 1992. Neutrophil adherence mediated by bpopolysaccharide in vitro. J. Clin. Invest. 90:2526–2535.

    Google Scholar 

  40. YOSHIDA, N., D. N. GRANGER, D. C. ANDERSON, R. ROTHLEIN, C. LANE, and P. R. KVIETYS. Anoxia/reoxygenation-induced neutrophil adherence to cultured endothelial cells. Am. J. Physiol. 262 (Heart Circ. Physiol. 31): H1891–1898, 1992.

    Google Scholar 

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Ohno, N., Ichikawa, H., Coe, L. et al. Soluble Selectins and ICAM-1 Modulate Neutrophil-Endothelial Adhesion and Diapedesis in Vitro. Inflammation 21, 313–324 (1997). https://doi.org/10.1023/A:1027349900279

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