Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatants of cytokine activated cultured endothelial cells

R Pigott; L P Dillon; I H Hemingway; A J Gearing

doi:10.1016/0006-291x(92)91234-h

Soluble forms of E-selectin, ICAM-1 and VCAM-1 are present in the supernatants of cytokine activated cultured endothelial cells

Biochem Biophys Res Commun. 1992 Sep 16;187(2):584-9. doi: 10.1016/0006-291x(92)91234-h.

Authors

R Pigott¹, L P Dillon, I H Hemingway, A J Gearing

Affiliation

¹ British Bio-technology, Oxford, U.K.

PMID: 1382417
DOI: 10.1016/0006-291x(92)91234-h

Abstract

Soluble forms of the adhesion molecules ICAM-1, VCAM-1 and E-selectin have been detected in supernatants from cytokine activated cultured endothelial cells. The release has been quantified using two site enzyme immunoassays. The molecular weights of the released molecules have been determined by immunoprecipitation and are consistent with the generation of soluble forms by cleavage at a site close to the point of membrane insertion.

MeSH terms

Cell Adhesion Molecules / analysis
Cell Adhesion Molecules / metabolism*
Cells, Cultured
Cytokines / pharmacology*
E-Selectin
Endothelium, Vascular / metabolism*
Enzyme-Linked Immunosorbent Assay
Humans
Immunosorbent Techniques
Intercellular Adhesion Molecule-1
Interleukin-1 / pharmacology
Molecular Weight
Solubility
Tumor Necrosis Factor-alpha / pharmacology
Vascular Cell Adhesion Molecule-1

Substances

Cell Adhesion Molecules
Cytokines
E-Selectin
Interleukin-1
Tumor Necrosis Factor-alpha
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1