With the new myocardial agent Tc-99m sestamibi, relatively high abdominal uptake represents a major limiting factor. The effect of a standard feeding (commercial milkshake taken immediately after injection), and posture (standing versus sitting for 10 minutes postinjection) on the resting biodistribution of sestamibi was investigated in patients receiving 3 to 5 MBq/kg injections as part of rest-stress tomography. Ancillary anterior 1-minute images of heart and abdomen were obtained at 15 minutes postinjection and 90 to 140 minutes postinjection in 32 patients, randomized to feeding and postural treatments. Feeding decreased the activity in the gallbladder at both 15 and 110 minutes, but had no effect on liver parenchyma; activity in a background rectangle immediately beneath the heart was decreased by feeding only on the 15-minute images. An effect of posture was not apparent. Further study of the acute effects of a milkshake in eight patients showed a prompt reduction of 26% in a subdiaphragmatic background rectangle, but a more gradual decline in gallbladder counts. Thus, feeding of lipid after injection is not an essential component of sestamibi imaging protocols; oral administration of fluid immediately before imaging may help reduce interfering gastric activity.