Diabetes mellitus is a multifactorial disorder characterised by hyperglycaemia and this leads to form advanced glycation endproducts (AGEs) and this cause diabetes complications. Plant extract of Momordica charantia is used worldwide as traditional medicines for the treatments of diabetes mellitus especially in developing countries. The aim of this study is to investigate the antiglycation and antioxidant effects of hot and cold water extracts of Momordica charantia on protein glycation. Advanced glycation endproducts formation and inhibition were monitored by a number of methods including sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), enzyme linked immunosorbent assay (ELISA), and fluorescent spectrometry. Furthermore, superoxide dismutase assay (SOD) and catalase assay (CAT) were used to study antioxidant properties of Momordica charatnia on bovine aortic endothelial cell in a cell culture model, The results have shown that hot and cold water extracts of Momordica charantia inhibit the production of AGEs in a dose-dependent manner. Additionally, the extraction efficiency of the hot water extracts was found to be highly influenced by temperature. Advanced glycation endproducts leads to creation of free radical species in all types of cells. These may contribute towards diabetic complications. Thus the aqueous extract of Momordica charantia, an edible vegetable, may have therapeutic potential in the management of diabetes mellitus.