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Cardiovascular magnetic resonance imaging: emerging techniques and applications
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  1. Amrit Chowdhary1,
  2. Pankaj Garg2,
  3. Arka Das1,
  4. Muhummad Sohaib Nazir3,
  5. Sven Plein1,3
  1. 1 Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, West Yorkshire, UK
  2. 2 Cardiovascular and Metabolic Medicine Group, University of East Anglia, Norwich, UK
  3. 3 School of Biomedical Engineering and Imaging Sciences, King's College London, London, UK
  1. Correspondence to Prof Sven Plein, Leeds, Yorkshire, UK; s.plein{at}leeds.ac.uk

Abstract

This review gives examples of emerging cardiovascular magnetic resonance (CMR) techniques and applications that have the potential to transition from research to clinical application in the near future. Four-dimensional flow CMR (4D-flow CMR) allows time-resolved three-directional, three-dimensional (3D) velocity-encoded phase-contrast imaging for 3D visualisation and quantification of valvular or intracavity flow. Acquisition times of under 10 min are achievable for a whole heart multidirectional data set and commercial software packages are now available for data analysis, making 4D-flow CMR feasible for inclusion in clinical imaging protocols. Diffusion tensor imaging (DTI) is based on the measurement of molecular water diffusion and uses contrasting behaviour in the presence and absence of boundaries to infer tissue structure. Cardiac DTI is capable of non-invasively phenotyping the 3D micro-architecture within a few minutes, facilitating transition of the method to clinical protocols. Hybrid positron emission tomography-magnetic resonance (PET-MR) provides quantitative PET measures of biological and pathological processes of the heart combined with anatomical, morphological and functional CMR imaging. Cardiac PET-MR offers opportunities in ischaemic, inflammatory and infiltrative heart disease.

  • cardiac magnetic resonance (CMR) imaging
  • cardiac imaging and diagnostics
  • advanced cardiac imaging
  • positron emission tomographic (PET) imaging

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Introduction

The indications for cardiovascular magnetic resonance (CMR) in international clinical practice guidelines continue to increase. CMR methods used in current clinical routine include anatomical imaging, cine imaging, flow velocity-encoded acquisition, angiography, dynamic and delayed contrast enhancement. Other CMR methods continue to transition into clinical routine, such as parametric mapping, which has recently been included in guidelines for the diagnosis of myocarditis. This review gives an overview of some other CMR methods that have the potential to move from research to clinical application in the near future.

Four-dimensional flow CMR

Blood flow in the heart is complex, dynamic and has a three-dimensional (3D) profile.1 Non-invasive imaging techniques, such as Doppler echocardiography and two-dimensional (2D) cine phase-contrast (PC) CMR are used in clinical routine to measure blood flow in the heart and vasculature, however, these methods cannot fully assess the complex, 3D and multidirectional nature of intracardiac blood flow.

Four-dimensional flow CMR (4D-flow CMR) is an emerging technology which allows time-resolved three-directional, 3D velocity-encoded PC imaging for 3D visualisation and quantification of valvular or intracavity flow2 (figure 1). Whole-heart 4D-flow CMR can be performed in under 10 min using retrospective ECG gating and free-breathing acquisition3 4 and several commercial solutions for data analysis are now available. These developments allow the inclusion of 4D-flow CMR in clinical protocols. The potential clinical applications of 4D-flow include qualitative assessment of complex flow patterns and vortices as well as quantitative measurement of valve regurgitation and kinetic energy (KE) (Table 1).

Figure 1

Overview of four-dimensional (4D)-flow scan. 4D-flow MRI involves acquiring three-directional velocity data in three-dimension (3D). These data can be then used offline to perform retrospective valve tracking to compute valvular flow. This method factors in valve motion, cardiac motion and allows direct regurgitant jet quantification as demonstrated in steps 3 and 4. 3D, three-dimensional; 4D, four-dimensional;CMR, cardiovascular magnetic resonance; FOV, field-of-view; FH, foot-head; RL,right-left; AP, anterior-posterior.

Table 1

Key principles, clinical translation, advantages, pitfalls and validation studies for 4D-flow, DTI and PET-MR

Visualisation of complex intracavity flow

4D-flow CMR data can be co-registered with cine images and displayed as two-directional vectors and colour coded with velocity information. Such an overlay allows novel insight into normal and abnormal flow patterns associated with cardiac disease. For example, an eccentric mitral regurgitation jet may be swirling in the entire left atrium and hence may not be limited to the defined backward flow seen by Doppler echocardiography. In addition, using 4D-flow CMR one can visualise flow accelerations through stenotic valvular or vascular lesions. This can provide complementary clinical information in challenging congenital heart disease cases where haemodynamic effects of anatomical abnormalities are not always clear. The visualisation of two-directional vectors may also enhance detection of septal defects, flow acceleration in outflow tracts and valvular regurgitation.

Vortex imaging

4D-flow CMR can help understand flow behaviour inside the cardiac chambers. One of the main mechanisms of intracardiac flow is vortex behaviour. Vortices can form in all chambers of the heart and in the major vessels. They can represent either optimal physiological flow5 or pathological flow due to dilatation6 or raised pressures.7 For example, vortical blood flow in the main pulmonary artery >14.3% of the cardiac interval corresponds to pulmonary hypertension with 97% sensitivity and 96% specificity. Similarly, in aortic root dilation, more helical flows with vortices can be identified with either 2D or 3D flow visualisation. Several quantification tools are under development to inform clinical management in aortopathy.

Quantification of 4D-flow

4D-flow CMR allows the measurement of several quantitative haemodynamic metrics. These include valvular flow, intracavity velocity or KE assessment, vortex quantification, blood flow component analysis and haemodynamic force quantification.8 According to a recent systematic review, the most clinically applicable technique is valvular flow quantification using retrospective valve tracking,8 while intracavity blood flow KE mapping shows promise.9

Retrospective valve tracking

Retrospective valve tracking using 4D-flow CMR allows quantification of flow through all four heart valves for the same averaged cardiac cycle, while factoring in valve motion by tracking the valve10 (figure 1). This method overcomes limitations that arise in conventional 2D CMR or echocardiographic flow imaging from physiological variation, throughplane motion and selection of the best imaging plane with more precise calculation of flows and intracardiac shunts. In this context, 4D-flow CMR can be considered to be the non-invasive ‘gold-standard’ for intracardiac flow quantification.11 In patients with limited echocardiographic views, 4D-flow CMR derived mitral inflow provides an alternative to assess peak E and A velocities for left ventricular (LV) diastolic function assessment. In addition, it can be applied to directly quantify regurgitant volumes by prescribing an analysis plane in the 4D volume data set that is exactly perpendicular to the regurgitant jet.12 Automated valve tracking procedures are becoming available which reduce postprocessing time significantly.13

Quantification of intracavity kinetic energy

Intracavity LV blood flow can be quantified for the complete cardiac cycle by 4D-flow CMR. This is achieved by superimposing endocardial contours defined from cine CMR images onto 4D-flow data. This process allows to quantify LV blood flow KE (figure 2). The KE of the blood represents a fundamental component of work performed by the heart which results in the movement of the blood. KE assessment factors in velocity in all the three directions (x, y and Z) per voxel. For each volumetric element (voxel), the formula for computing KE is KE=1/2 ρblood.Vvoxel.v2 where ρblood is the density of blood (1.06 g/cm3), Vvoxel is the voxel volume and v the velocity magnitude. For each cardiac phase, the total KE within the LV is obtained by summation of the KE of every voxel. The analysis of KE is largely automated and a key strength of this technique is its excellent intraobserver/interobserver agreement (coefficient of variation=6%±1%) and almost immediate haemodynamic assessment without requiring additional segmentations to those acquired in routine practice.8 Importantly, as it factors in the complete intracavity flow, LV blood flow KE is a finer marker of diastolic function than standard diastolic parameters.8 Also, other studies have shown how it independently predicts adverse remodelling postmyocardial infarction (MI)14 and LV thrombus formation.15 Therefore, this emerging tool for haemodynamic assessment holds great promise for phenotyping cardiac haemodynamics, informing diagnosis and potentially predicting disease course. Future trials are needed to evaluate its value in informing treatment outcomes in cardiovascular diseases.

Figure 2

Four-dimensional (4D) flow cardiovascular magnetic resonance allows reliable and instant quantification of left ventricular blood flow kinetic energy for haemodynamic assessment. Left ventricular (LV) kinetic energy (KE) curves changes with age and sequentially to the degree of LV impairment. This figure demonstrates LV KE maps at three crucial stages of cardiac cycle—peak systole (yellow), peak early filling (green) and late filling (blue). EF, ejection fraction; LVEDV, left ventricular end-diastolic volume; LVESV, left ventricular end-systolic volume; MI, myocardial infarction.

Clinical potential of 4D-flow

Four-dimensional flow imaging with CMR has reached a level of technical maturity that allows it to migrate from research to clinical application. Acquisition times of under 10 min are achievable for a whole heart multidirectional data set and commercial software packages are available for data analysis. However, standardisation and validation of acquisition and analysis protocols and large-scale evidence is needed to define its future role. Postprocessing remains time-consuming and automisation of at least some analysis steps will be essential to facilitate clinical translation. The first clinical applications of 4D-flow CMR are likely to be in assessment of aortic and complex congenital heart disease (table 1).

Diffusion tensor imaging

Diffusion tensor imaging (DTI) is already in clinical use outside of the heart and has the unique ability to characterise microstructural tissue changes. Application of this method for cardiac imaging has been challenging, but in recent years cardiac DTI methods have shown clinical potential.

DTI is based on the measurement of molecular water diffusion and uses contrasting behaviour in the presence and absence of boundaries to infer tissue structure (table 1). Where there are no boundaries, water molecules diffuse freely, while in the presence of physiological barriers (such as cell membranes, capillaries and extracellular matrix) this free diffusion is restricted. DTI can be used to probe the primary directions and magnitudes of diffusion.16–18 Cardiac DTI is capable of non-invasively phenotyping the 3D micro-architecture of the heart.19 In addition, by acquiring data in multiple phases of the cardiac cycle, DTI can provide dynamic information and improve understanding of myocardial laminar structure and its complex inter-play with functional mechanics.

For DTI reconstruction, diffusion is measured in at least six different directions, which enables the mathematical description of diffusion using tensor notation; pictorially, tensors can be described using ellipsoids whose primary axis coincides with the primary diffusion direction.20 Based on the derived tensor representation, it is possible to infer in each voxel a number of different tissue characteristics. The molecular diffusion rate or mean diffusivity (MD) is a measure of how freely water can diffuse in the myocardium, while the degree of diffusion restriction in different directions is measured using fractional anisotropy (FA) (figure 3). The underlying tissue structure orientation is described using the eigenvectors of the diffusion tensor. These lead to commonly reported measures such as the helix angle (HA) and second eigenvector angle (E2A) (figure 4). The HA is a measure of the orientation of cardiomyocytes, which changes transmurally from left-handed at the epicardium, to circumferential in the midwall and right-handed at the endocardium. HA maps depict the average orientation of cardiomyocytes on a voxel-wise basis. E2A describes the angulation of laminar sheetlets in the myocardial wall (the angle between the cross-myocyte eigenvector E2 and radial direction), and values can also be plotted on a map to depict the local contractile state of the myocardium.

Figure 3

Illustration of the degree of diffusion restriction in different directions measured using fractional anisotropy (FA). In voxels consisting of coherently aligned myocytes, the FA is expected to be high whereas it is expected be low in voxels with disorganised myocyte orientation such as hypertrophic cardiomyopathy. Reproduced with permission from Tarkin et al.42 ECV, extracellular volume; HCM, hypertrophic cardiomyopathy.

Figure 4

Diagram illustrating the calculation of helix angle (HA) and second eigenvector angle (E2A) in each voxel. (A) Illustration of three cubic slices of non-contiguous voxels from the subendocardial, midwall and subepicardial layers. (B) HA is calculated between the circumferential direction and projection of the primary eigenvector. (C) The cubes are sectioned perpendicular to the E1 proj to calculate the cross-myocyte components of diffusion. (D) The E2 angle is measured between the secondary eigenvector projection and cross-myocyte direction in the wall tangent plane. Reproduced with permission from Khalique et al.32

DTI has been shown to be reproducible in studies involving both healthy and diseased cohorts.20–22 Furthermore, in healthy volunteers regional heterogeneity of DTI parameters mentioned above have been demonstrated as well as the direct relationship between the E2A and HA gradients with LV mass and body surface area.23 An inverse relationship between E2A mobility and both age and ejection fraction of the LV has been described.24 These indices have been used in ex vivo and in vivo studies of healthy and diseased myocardium (figure 5).

Figure 5

Multiparametric imaging of the left ventricle myoarchitecture and disarray using diffusion tensor imaging (DTI) and late gadolinium enhancement (LGE). Column A displays a typical basal left ventricular (LV) slice obtained in a healthy control showing a normal mean diffusivity (MD), fractional anisotropy (FA), second eigenvector angle (E2A) and helix angle (HA). Column B demonstrates a basal LV slice in a patient with myocardial infarction (MI) with inferior LGE, raised MD and low FA. Column C demonstrates a basal LV slice in a patient with hypertrophic cardiomyopathy (HCM) with a raised MD and E2A and a low FA. Column D demonstrates a basal LV slice in a patient with dilated cardiomyopathy (DCM) demonstrating a raised MD and low FA and E2A.

Myocardial infarction

Following acute MI, myocardial necrosis and oedema lead to disruption of the myocardial micro-architecture in the infarct zone and in the peri-infarct tissue. DTI studies have demonstrated an increased MD and a decreased FA in the acute infarct zone when compared with remote myocardial segments.25 26 Tractographic propagation angle (PA), which is a newer metric of cardiac myofibre architecture derived from DTI, has been used to delineate regions of infarct and shows good correlation with late gadolinium enhancement (LGE) imaging without the need for contrast agents.27 Gaining a better understanding of the microstructural effects of myocardial ischaemia and infarction using DTI should help elucidate the intricacies of the pathophysiological processes that lead to adverse LV remodelling.

Hypertrophic cardiomyopathy

Myocardial hypertrophy that is not solely explained by abnormal loading conditions and myofibre disarray are hallmarks of hypertrophic cardiomyopathy (HCM). Using DTI, the presence of myofibre disarray and dynamic myocardial microstructure alterations in the cardiac cycle have been demonstrated; while E2A and MD are elevated, FA is low in patients with HCM when compared with controls.28 29 FA has also been studied as a marker of disarray, with the potential to identify patients at risk of ventricular arrhythmias and sudden cardiac death.29

Dilated cardiomyopathy

Ventricular chamber enlargement, systolic dysfunction and LV wall thinning are characteristic findings in dilated cardiomyopathy (DCM). A reduced systolic E2A, sheetlet mobility and FA, along with raised MD have been demonstrated in patients with DCM when compared with controls.30 31 In contrast to healthy controls, the change in HA from diastole to systole was far less pronounced in DCM and the diastolic myofibre arrangement had a more longitudinal orientation.31 It is anticipated that an assessment of myocardial microstructure will provide a new tool for risk-stratification of patients with DCM.

Congenital heart disease

In congenital heart disease (CHD), significant derangement of myofibre orientation has been demonstrated in situs inversus totalis using DTI.32 A predominance of longitudinal and oblique fibres in the systemic right ventricle hypothesised to be an adaptive response to systemic pressure and load has been demonstrated by DTI.33 DTI is expected to improve understanding of the complex effect of CHD on the myocardial microstructure with potential impact on clinical care.

Clinical potential of cardiac DTI

Although at an early stage of its clinical development, cardiac DTI offers the unique potential of providing a non-invasive window into the microstructure of the heart, previously only accessible with invasive myocardial biopsy. Cardiac DTI images can now be acquired within a few minutes, making integration into clinical studies a realistic option. While data analysis remains time consuming and limited to specialist centres and consensus on the most robust DTI-derived measurements is pending, these current limitations are likely to be overcome within the next years. As for 4D-flow, standardisation of acquisition and analysis as well as faster, partly automated postprocessing will be required before the method transcends into clinical practice. Artificial intelligence will provide some of the necessary technical developments and the Society for Cardiovascular Magnetic Resonance is promoting standardisation of methods. The anticipated clinical use of cardiac DTI is in cardiomyopathies and congenital heart disease, but other applications are likely to emerge (table 1).

Positron emission tomography-magnetic resonance

Hybrid positron emission tomography-magnetic resonance (PET-MR) systems have recently been introduced and are finding clinical applications in many body systems, with cardiovascular indications beginning to emerge. Cardiac PET provides quantitative measures of biological and pathological processes of the heart, while CMR derives anatomical, morphological and functional imaging. The combined assessment of PET and CMR offers opportunities in ischaemic, inflammatory and infiltrative heart disease (table 1).

Ischaemic heart disease

Viability

PET-MR has potential to more accurately predict functional recovery than PET or CMR alone by providing an assessment of the metabolic activity of cells from 18F-fluorodeoxyglucose (18F-FDG), with tissue perfusion assessment by PET or CMR, scar imaging with LGE CMR and functional reserve with low-dose dobutamine CMR. This combined information may be particularly relevant in patients with intermediate scar extent of 25%–75% transmurality where the specificity of LGE CMR alone is low for prediction of functional recovery. In a small study of patients scheduled for coronary revascularisation, 18F-FDG PET-MR reclassified 19% of non-assessable segments with intermediate extent of LGE.34 Another study demonstrated a poor correlation between hibernating myocardium defined by perfusion/viability mismatch with 13N-Ammonia/18F-FDG PET and LGE with CMR, although did not use low-dose dobutamine stress CMR, which suggests that both modalities may confer synergism.35 Larger clinical studies and outcome data are required to determine the incremental prognostic benefit of PET-MR in hibernating myocardium.

Coronary imaging

PET-MR allows combined assessment of coronary anatomy and metabolism. There are emerging CMR methods to obtain high-resolution isotropic coronary images in short scan times.36 Using PET-MR, advanced reconstruction methods with non‐rigid respiratory and cardiac deformation fields estimated from CMR can be used to motion-correct CMR angiography and 18F-FDG data.37 In a pilot study, 18F-fluoride was found to be a marker of high-risk vulnerable plaque localised by CMR38 and a recent study has demonstrated good agreement between quantitative PET-CT and PET-MR data.39 Future studies may obtain radiation-free coronary imaging alongside motion-corrected PET data to localise and identify metabolically active foci indicative of vulnerable coronary plaque prior to adverse cardiac events.

Inflammatory heart disease

Active inflammatory myocardial cells have greater glucose utilisation which makes 18F-FDG attractive to evaluate inflammation.40 In a PET-MR study of patients with suspected myocarditis, PET and CMR provided complementary data and showed good agreement between 18F-FDG and T2-weighted CMR and LGE.41 Emerging radiotracers such as 68GA-DOTATATE are sensitive to macrophage activity,42 which may be co-registered with areas of T2 mapping with CMR to detect early disease prior to development of fibrosis.

The diagnosis of cardiac sarcoidosis is challenging and both PET or CMR are recommended in current guidelines.43 Several small studies have confirmed the technical feasibility of hybrid PET-MR in suspected cardiac sarcoidosis. These have suggested different patterns of either 18F-FDG uptake or LGE or both,44 with sometimes poor agreement between the two methods, suggesting they track different pathology.45 Thus, PET-MR may provide complementary data with active metabolic inflammation from PET alongside fibrosis from CMR, which can lead to new classifications of the presence and activity of cardiac sarcoidosis46 (figure 6).

Figure 6

Positron emission tomography-magnetic resonance (PET-MR) images in a patient with suspected inflammatory heart disease in the short axis orientation. Panel A: reformatted three-dimensional (3D) multiplanar images of isotropic whole heart late gadolinium enhancement (LGE) imaging. There is near circumferential midwall myocardial enhancement of the anteroseptum, inferoseptum, extending to the inferior wall (arrowed). Panel B: image fusion following co-registration of PET and CMR images, demonstrates 18F-fluorodeoxyglucose myocardial uptake in the anteroseptum and inferolateral wall (arrowed) closely corresponding to midwall LGE. Using hybrid imaging and novel 3D whole heart LGE methods allow for closer registration of data and enhanced motion correction of PET data. An important differential is cardiac sarcoidosis, and hybrid PET-MR may have potential role for diagnosis of patients with cardiac sarcoidosis. Images courtesy of Eliana Reyes, King’s College London.

Future PET-MR studies with novel inflammation tracers in patients with well-characterised phenotype of cardiac sarcoidosis may provide mechanistic insights into disease process, improve diagnosis and guide anti-inflammatory therapy.

Infiltrative heart disease

CMR has clinical utility for cardiac amyloidosis with T1 parametric mapping, extracellular volume and LGE. From a nuclear medicine perspective, 99mTc-3,3-diphosphono-1,2-propanodicarboxylic acid bone scintigraphy can be used to accurately differentiate between AL and ATTR amyloid and provide semi-quantitative markers of amyloid burden.47 One PET-MR study demonstrated greater 18F-fluoride uptake in areas of LGE in patients with ATTR amyloid compared with AL amyloid and healthy controls using PET-MR.48 Other emerging PET tracers have been shown to detect cardiac amyloidosis such as 18F-florbatepen49 and 11C-Pittsburgh compound B,50 with potential to provide absolute quantitative measures of amyloid burden. Future studies may investigate these tracers and co-register and motion-correct the PET data with CMR data to provide quantitative markers of amyloid burden, which may be used to monitor response to chemotherapeutic agents.

Cardiac masses

PET-MR has been used to investigate cardiac masses using 18F-FDG, although without parametric mapping or first-pass perfusion as part of the CMR protocol.41 The absolute clinical utility was limited, since there was a high diagnostic performance of standalone PET and CMR. However, PET-MR may provide additional information in cardiac masses that standalone imaging cannot fully characterise and potentially guide intracardiac biopsy.

Clinical potential of PET-MR

Where available, cardiac PET-MR can already be integrated into clinical workflows when evidence for the individual use of PET and CMR is available (Table 1). As outlined above, the most likely clinical use is in inflammatory and infiltrative heart disease and in the assessment of cardiac masses. However, access to PET-MR is currently limited to a few specialist centres, with wider availability required for the method to become a common clinical tool. Developments of novel radiotracers, sequence development, attenuation correction, co-registration, motion correction and postprocessing with dedicated software for image fusion will further expand its clinical potential to gain mechanistic insight into disease, improve diagnoses, develop novel imaging biomarkers, guide therapeutics and provide risk stratification.

Conclusions

4D-flow, DTI and PET-MR are examples of the recent advances in CMR that are crossing the boundary from research to clinical practice. They promise better understanding of disease mechanisms and the potential for improved disease detection and risk stratification. Key challenges are acquisition time for complex methods and standardisation and, in particular in the case of PET-MR, limited access to the required hardware. As these challenges are overcome, it is likely that CMR methods in current development will become available for clinical use.

References

Footnotes

  • Twitter @DrAChowdhary, @sohaibnazir, @Sven Plein

  • Collaborators Christopher I Kelly.

  • Contributors All authors have contributed to literature search and writing of the manuscript.

  • Funding SP is funded by a British Heart Foundation Chair (CH/16/2/32089).

  • Competing interests None declared.

  • Patient and public involvement Patients and/or the public were not involved in the design, or conduct, or reporting, or dissemination plans of this research.

  • Provenance and peer review Commissioned; externally peer reviewed.

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