Objective To examine the circadian variation in the signal averaged electrocardiogram (saECG) and heart rate variability and investigate their relations in healthy subjects.
Methods 24 hour ECGs were obtained with a three channel recorder using bipolar X, Y, and Z leads in 20 healthy subjects. The following variables were determined hourly: heart rate, filtered QRS (f-QRS) duration, low and high frequency components of heart rate variability (LF and HF), and the LF/HF ratio.
Results Heart rate, f-QRS duration, HF, and the LF/HF ratio showed significant circadian rhythms, as determined by the single cosinor method. Heart rate and the LF/HF ratio increased during daytime, and f-QRS duration and HF increased at night. f-QRS duration was negatively correlated with heart rate (r = 0.95, p < 0.001) and the LF/HF ratio (r = 0.94, p < 0.001) and positively with HF (r = 0.93, p < 0.001).
Conclusions f-QRS duration has a significant circadian rhythm in healthy subjects and is closely related to the circadian rhythm of autonomic tone.
- signal averaged ECG
- f-QRS duration
- circadian rhythm
- heart rate variability
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Signal averaged electrocardiography (saECG) is a non-invasive method of detecting the substrate for malignant ventricular arrhythmias and is widely used to identify individuals at high risk for such arrhythmias and for sudden cardiac death.1-5 Although the saECG has been assumed to measure fixed parameters, several studies have shown that these are altered by changes in factors such as heart rate and autonomic tone.6-8 Analysis of heart rate variability has been used to evaluate cardiac autonomic tone.9-11 Heart rate, heart rate variability, cardiac autonomic tone, and ventricular arrhythmias are known to have a circadian rhythm,12-16 but information on the possibility of a circadian rhythm of the saECG and on the contribution of circadian fluctuations in autonomic function to the saECG is limited. We investigated whether the saECG has a circadian rhythm in healthy subjects and examined the relation between circadian fluctuations of the saECG and heart rate variability.
We analysed 24 hour ECG recordings obtained in 44 healthy volunteers (28 men and 16 women, mean age (SD): 28.2 (3.3) years) in whom no abnormalities were detected by the medical history, physical examination, biochemical profile, 12 lead ECG, echocardiogram, or chestx ray. No subjects were on drug treatment.
ANALYSIS OF 24 HOUR ECG RECORDINGS
The 24 hour ECG recordings were made with a three channel recorder (model 459, Del Mar Avionics, Irvine, California, USA) using bipolar leads X, Y, and Z.
Signal averaging was performed on the data obtained from the first 500 seconds of every hour over the 24 hour period and filtered with a bidirectional filter at 40 to 250 Hz. The filtered leads were combined to form a vector as: . The QRS onset and offset were determined by a computer algorithm. The computer determined QRS offset point was checked visually and if incorrect, the actual QRS offset point was determined manually. If the terminal portion of the QRS—which can be affected by the noise level—was too small to permit accurate determination, the data were excluded from analysis. Data with a noise level greater than 1 μV were also excluded from analysis.
Heart rate variability analysis
The power spectrum of the RR intervals was computed by a fast Fourier transformation and expressed as the square root of the areas under the power spectrum. We calculated the power of the low frequency component (LF, 0.04 to 0.15 Hz) and the high frequency component (HF, 0.15 to 0.40 Hz) for the first 300 seconds of every hour over the 24 hour period and analysed the mean hourly values. The ratio of the LF to HF components (LF/HF) was also determined for the first 300 seconds of each hour. Measures of heart rate variability were transformed by a natural logarithm because their distributions were skewed. We selected only data in which the heart rate, the baseline of ECGs, and the configuration of QRS-T waves were stable for at least 10 minutes, which suggests the absence of any abrupt change in posture or autonomic activity. We excluded data from 24 subjects because the saECG and heart rate variability data were available for less than 21 of 24 hours. Thus we analysed data obtained in the remaining 20 subjects.
The results are means (SD) unless otherwise specified. Heart rate, heart rate variability indices, and the filtered QRS (f-QRS) duration from the saECG were averaged on an hourly basis. The hourly mean data were analysed by the single cosinor method,17 in which a least squares procedure is used and a cosine function is fitted to the data. This model is characterised by three parameters: (1) the mesor, the rhythm determined average; (2) the amplitude, one half of the difference between the highest and the lowest values in a rhythm defined by the cosine function; and (3) the acrophase, the lag from the reference time point (midnight) to the time of the highest value in the cosine model fitted to the data. The significance of the circadian rhythms was evaluated by the zero amplitude test.
The relation between f-QRS duration and heart rate and heart rate variability was evaluated by linear regression analysis using hourly mean group data and hourly data obtained in individual subjects. Data were analysed by the Student’s t test. A p value < 0.05 was considered statistically significant.
CIRCADIAN RHYTHM OF HEART RATE, HEART RATE VARIABILITY, AND f-QRS DURATION
Heart rate, HF, the LF/HF ratio, and f-QRS, but not LF, showed significant circadian rhythms (fig 1). HF and f-QRS duration increased at night and decreased during the day, whereas heart rate and the LF/HF ratio were greater during the day than at night. The chronobiological parameters characterising the circadian rhythms of the variables studied are summarised in table 1. The acrophases of HF and f-QRS duration were similar, as were those of heart rate and the LF/HF ratio.
Figure 2 shows the saECG recordings obtained in a 24 year old healthy woman at 05:00 and 11:00.
CORRELATION BETWEEN f-QRS DURATION AND HEART RATE VARIABILITY
f-QRS duration was negatively correlated with heart rate (r = 0.95, p < 0.001) and the LF/HF ratio (r = 0.94, p < 0.001), and positively with HF (r = 0.93, p < 0.001) (fig 3). f-QRS duration was not correlated with LF. When the hourly data in individual subjects were analysed, f-QRS duration was positively correlated with LF in eight of 20 subjects (40%) and with HF in 17 (85%), and negatively correlated with heart rate in all subjects and with the LF/HF ratio in 18 (90%). In none of the subjects was f-QRS duration negatively correlated with LF or HF, or positively correlated with heart rate or the LF/HF ratio.
Healthy subjects showed a significant circadian rhythm in the f-QRS duration of the saECG that was closely related to the circadian rhythm of heart rate variability. f-QRS duration decreased during the day and increased at night.
Spectral analysis of heart rate variability is used for quantitative assessment of autonomic activity. There is general agreement that the power of the HF oscillation, which is related to respiration, is a marker of efferent vagal input to the heart, and that the LF/HF ratio is a marker of sympathetic tone. LF power is an indicator of the sympathovagal balance.9-11 It is well established that heart rate and heart rate variability have a circadian rhythm.12-14 We evaluated the f-QRS duration as a function of heart rate variability during routine daily activity. f-QRS duration showed a significant negative correlation with the heart rate and the LF/HF ratio, and a significant positive correlation with HF, suggesting that the circadian variation of the f-QRS duration is closely related to the circadian fluctuation in heart rate or autonomic nerve activity, or both. Previous studies have shown that atrial pacing has no effect on saECG parameters in patients with coronary artery disease or a history of ventricular tachycardia or cardiac arrest.18-20 Infusion of isoprenaline7 and exercise6 or tilt7 induced sympathetic activation shorten the f-QRS duration. Thus the shortening of f-QRS duration during the daytime may not have been related to the increase in heart rate itself, but rather to increased sympathetic activity or decreased parasympathetic activity.
One of the most important determinants of QRS duration is ventricular conduction. Munger et al showed that β adrenergic modulation of cardiac conduction was voltage dependent and that isoprenaline shortened the conduction time at the normal resting potential, but caused additional slowing of conduction in depolarised canine Purkinje fibres.21 Ono et al reported that isoprenaline increased or decreased the sodium current in canine, rabbit, and guinea pig myocytes depending on the experimental conditions of the holding and the test potential.22 The effect of β adrenergic stimulation on ventricular conduction in vivo remains to be studied. Our data suggest that increased sympathetic tone or decreased parasympathetic tone, or both, enhances ventricular conduction during routine daily activity in healthy subjects.
An important limitation of this study is the noise level of the saECG owing to the use of Holter monitoring. The noise level may influence determinations of the f-QRS duration.23 We had to exclude 24 subjects from the analysis because of relatively high noise levels. However, the alteration in the f-QRS duration in response to changes in the heart rate or heart rate variability was greater than the expected change caused by differences in the noise level.
The saECG is a useful non-invasive test for identifying areas of slowed conduction that are a prerequisite for reentrant ventricular arrhythmias.1-5 Although the substrate or late potential identified by the saECG has been assumed to be fixed, our results suggest that the saECG has a circadian rhythm and may alter with changes in the autonomic tone. However, we examined only young healthy subjects. Further studies are needed to evaluate the circadian rhythm of the saECG and the effects of autonomic tone on saECG in patients with heart disease.