OBJECTIVE To explore whether the anaerobic threshold, a measure of the balance between aerobic and anaerobic cellular metabolism, is related to whole body insulin sensitivity in healthy individuals and in patients with chronic heart failure, which involves is an imbalance of aerobic and anaerobic metabolism.
DESIGN Case–control study.
SETTING A teaching hospital department specialising in heart failure.
PATIENTS 20 healthy individuals (mean (SEM) age 55.2 (2.7) years) and 36 patients with chronic heart failure (59.1 (2.0) years, New York Heart Association class I–IV, anaerobic threshold 11.8 (0.7) ml/kg/min, left ventricular ejection fraction 26 (2)%).
INTERVENTIONS An intravenous glucose tolerance test for assessment of insulin sensitivity (minimal model analysis) and a maximum treadmill exercise test for assessment of the anaerobic threshold, derived from measurement of oxygen consumption and carbon dioxide output.
MAIN OUTCOME MEASURES Relation between insulin sensitivity and the anaerobic threshold in patients with chronic heart failure.
RESULTS While anaerobic threshold was positively correlated with insulin sensitivity in healthy controls (r = 0.72, p < 0.001), no such relation was observed in patients with chronic heart failure. In stepwise multiple linear regression analyses of variables in healthy individuals, insulin sensitivity emerged as the only predictor of anaerobic threshold (standardised coefficient = 0.72, p < 0.001), while fasting insulin, incremental insulin area, and total body fat (dual photon x ray absorptiometry) failed to enter into final models (jointR = 0.52, p < 0.001).
CONCLUSIONS In healthy individuals, whole body insulin sensitivity is related, or “coupled,” to the anaerobic threshold. The absence of such metabolic coupling in patients with chronic heart failure provides further evidence of disturbed cellular metabolism in patients with this condition.
- heart failure
- anaerobic threshold
- insulin resistance
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The cellular supply of energy in the form of high energy phosphates relies on a balance between glycolysis (mainly anaerobic) and the citric acid cycle (aerobic). As well as requiring glucose and oxygen, the generation of glycolytic intermediates also requires insulin. This hormone not only drives cellular uptake of glucose, but also exerts a regulatory influence on glycolytic enzymes.1-3 Thus cellular metabolism requires coordination or “coupling” between the cellular processing of glucose and oxygen and insulin action.
On the basis of the above, we considered that whole body measures of the anaerobic threshold, which reflects the balance between aerobic and anaerobic metabolism, might relate to whole body insulin sensitivity. Our investigation focuses on healthy individuals and on patients with chronic heart failure—an insulin resistant state4 in which there is an imbalance between oxidative and non-oxidative metabolism.5-8
The study group consisted of 36 patients with chronic heart failure caused by coronary heart disease (19) or dilated cardiomyopathy (17). The diagnosis of chronic heart failure was based on standard criteria. All patients had been in heart failure for at least six months before the study. Concurrent treatment in the heart failure group included angiotensin converting enzyme inhibitors, loop diuretics, digoxin, warfarin, nitrates, amiodarone, and thiazide diuretics, either alone or in combination. No patients were taking hypouricaemic drugs. Healthy controls were matched for age and sex and were free from cardiovascular disease, on the basis of clinical history and examination and routine investigations. All patients gave written, informed consent and the study was approved by the local ethics committee.
During cardiopulmonary exercise testing, all patients were exercised to exhaustion (respiratory exchange ratio > 1.1). A one way valve connected to a respiratory spectrometer (Amis 2000, Odense, Denmark) was employed. Oxygen consumption was calculated on-line using a standard inert gas dilution technique.9 ,10 The slope of the regression line relating minute ventilation to carbon dioxide output was employed as an index of the ventilatory response to exercise.11 These measures were obtained during a maximum exercise test, using a modified Bruce protocol for patients with chronic heart failure.
The ventilatory anaerobic threshold was calculated according to the technique of Beaver et al.12Briefly, the anaerobic threshold is selected as the oxygen consumption (V˙o 2) at which the slope of the carbon dioxide output (V˙co 2) versusV˙o 2 changes from 1 or slightly less than 1 to a slope which is steeper than 1. The intersection of these slopes is taken as the V˙o 2 above which the increased V˙co 2 can only be explained by the increase in metabolic acidosis.
Participants underwent an intravenous glucose tolerance test with sampling at 16 time points during 180 minutes, as previously described.13 Dextrose was given intravenously as a 50% solution, at a dose of 0.5 g/kg body weight. Plasma glucose was determined on the same day using glucose oxidase procedures.14 Plasma insulin concentrations were measured on samples stored at −20°C using a radioimmunoassay procedure.15 Insulin sensitivity, inversely related to insulin resistance, was assessed using the minimal model approach.16
BODY FAT COMPOSITION
Height (m) and weight (kg) were determined to calculate body mass index (BMI: weight/height2). Total fat mass was estimated by dual energy x ray absorptiometry (DXA) using a Lunar DPX (Lunar Corporation, Madison, Wisconsin, USA).17 All scans were performed and analysed using version 3.6z software. Precision of fat tissue measurements was better than 5%.18
These were carried out using the SYSTAT statistical package (SYSTAT Inc, Evanston, Illinois, USA). Univariate Pearson correlation coefficients were derived. A general linear model was used in multivariate analyses of pooled subjects. In all analyses, a p value of < 0.05 was considered statistically significant.
As shown in table 1, the chronic heart failure group had a lower insulin sensitivity (p = 0.035) and anaerobic threshold (p < 0.001) as well as lower systolic (p = 0.002) and diastolic (p = 0.001) blood pressures.
Univariate analyses of variables in healthy individuals revealed a significant correlation between anaerobic threshold and insulin sensitivity, incremental insulin area, and fasting insulin (table2). In contrast, no significant correlations were observed in the chronic heart failure group (fig 1).
Variables which were found to be correlated in univariate analyses were entered in multivariate analyses. In stepwise multiple linear regression analyses of variables in healthy individuals, insulin sensitivity emerged as the only predictor of anaerobic threshold (standardised coefficient = 0.72, p < 0.001), while fasting insulin, incremental insulin area, and total body fat failed to enter into final models (joint R = 0.52, p < 0.001).
We have shown that in healthy individuals, there is a strong relation between the anaerobic threshold and insulin sensitivity. This relation might be expected in view of the coordination that exists between oxygen and glucose uptake at the cellular level. A salient finding from this study is that the relation between the anaerobic threshold and insulin sensitivity observed in healthy individuals is absent in patients with chronic heart failure.
Our demonstration of a lower anaerobic threshold in patients with chronic heart failure is in keeping with the finding of an early switch to anaerobic metabolism in this condition, as evidenced by a rapid rise in blood lactate levels during exercise.6 ,7 ,19 In contrast to our findings in healthy individuals, we found that in patients with chronic heart failure, reductions in insulin sensitivity are not accompanied by commensurate reductions in the anaerobic threshold. This suggests that the coupling between glucose and oxygen metabolism and insulin action that occurs in healthy individuals is disturbed in chronic heart failure.
Several factors may be responsible for the lack of this relation.
First, it is recognised that insulin may, by causing vasodilatation, promote its own transport into skeletal muscle—the principal site of insulin action.20 On the other hand, insulin mediated vasodilatation is impaired in states of insulin resistance.20 Thus the impairment in peripheral vascular responses observed in patients with chronic heart failure21-24 might not only lead to variation in the tissue availability of oxygen but also in the delivery of insulin to skeletal muscle. This could be responsible for the loss of a relation between insulin sensitivity and the anaerobic threshold.
Second, chronic heart failure involves disturbances in oxidative and lipolytic enzymes as well as in skeletal muscle fibre distribution and mitochondrial structure,25 which could contribute to disturbances in the tissue responsiveness to insulin.
Third, defective post-receptor insulin signalling, arising from reductions in the glucose transporter protein GLUT-4 found in the myocardium of patients with heart failure,26 may also contribute to tissue responsiveness to insulin.
We conclude that whereas in healthy individuals, the anaerobic threshold is positively related to insulin sensitivity, this relation is absent in patients with chronic heart failure. This observation is in keeping with the known mechanisms that link oxygen and glucose metabolism and insulin action at the cellular level. On the basis of our findings, disturbances in cellular metabolism that occur in heart failure, such as a low anaerobic threshold, do not appear to be related to reductions in insulin sensitivity. Further studies are needed to determine which additional factors are responsible for the “uncoupling” of glycolysis and insulin mediated glucose uptake in patients with chronic heart failure.
FL and IFG were supported by the Heart Disease and Diabetes Research Trust and by the Rosen Foundation.