Article Text
Abstract
Objective To investigate the effects of CT-1 on differentiation of induced swine BMSCs in vitro independently or with 5-aza.
Methods BMSCs were divided into four groups : bland contro1; induced with CT-1; induced with 5-aza; induced with 5-aza combined with CT-1. After 4 weeks of induced culturing, the differentiation of cardiomyocyte induced were estimated by cTnT and a-actinin.
Result Red fluorescence staining of a-actin shows: the differentiation rate of myocardial cells of the composite group is greatest (29.900±4.7571%); 5-aza group is greater than CT-1 group; CT-1 group is greater than control. Red fluorescence staining of cTnT shows: the differentiation rate of myocardial cells of the composite group is greatest (36.500±4.0927%); 5-aza group is greater than CT-1 group; CT-1 group is greater than control.
Conclusions CT-1 can increasing the inducing rate commbined witn 5-aza.