Objectives To explore the effects of Lisinopril upon the activities of Na+, K+-ATPase and Ca2+-ATPase and mRNA expression levels of Na+, K+-ATPase a1-subunit and plasma membrane Ca2+-ATPase isoform 1 (PMCA1) in cultured thoracic aorta vascular smooth muscle cells (ASMCs) isolated from spontaneously hypertensive rats (SHR).
Methods ASMCs were divided into four groups: Wistar-Kyoto (WKY) control, SHR control, Lisinopril (1×10−5) intervened SHR group and Lisinopril (1×10−6) intervened SHR group. The activities of ion pumps were detected by spectrophotography and mRNA expressions were measured by real time PCR. The content of Angiotensin II (Ang II) in cells-cultured medium were detected by radioimmunoassay.
Results The activities of Na+, K+-ATPase, Ca2+-ATPase and the mRNA expression levels of Na+, K+-ATPase a1-subunit and PMCA1 in ASMCs from SHR were significantly lower than those from WKY control (p<0.01). Lisinopril significantly increased the activities of Na+, K+-ATPase and Ca2+-ATPase and mRNA expression levels of Na+, K+-ATPase a1-subunit and PMCA1 in ASMCs from SHR (p<0.01). Ang II content of culture medium in ASMCs from SHR was significantly more than those from WKY control (p<0.05), Lisinopril attenuated Ang II content of ASMCs culture medium from SHR (p<0.05).
Conclusions The decreased activities of Na+, K+-ATPase and Ca2+-ATPase may be related to their lower expression of the mRNA in ASMCs from SHR. The Lisinopril may increase the activities of two ion pumps and upregrulae the mRNA expression of Na+, K+-ATPase a1-subunit and PMCA1 in ASMCs from SHR through blocking the generation of Ang II.
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