Objective The aim of this study was to explore the possible association of the thrombospondin-4 (TSP-4) gene G29926C (A387P) polymorphism with acute coronary syndrome (ACS) in a Chinese Han population.
Methods The TSP-4 A387P polymorphism was determined by PCR and restriction fragment length polymorphism (RFLP) analysis. A total of 749 unrelated subjects were included in the study, consisted of two groups: the ACS group and control group. The ACS group was composed of 412 patients with ACS (298 men and 114 women) recruited from hospitalised patients at four participating hospitals between November 2003 and May 2006. The diagnosis of ACS was based on the diagnostic criteria of 2002. AHA/ACC guideline. The control group consisted of 337 age- and sex- match subjects (232 men and 105 women) who were judged to be free of CAD by history, clinical examination, electrocardiography, exercise test and angiography, which were selected from inpatients at the same hospitals. Subjects with cardiomyopathy, tumour, and renal or hepatic disease were excluded from the study.
Results Slightly decreased frequency of GC genotype was observed in patients with ACS, compared with controls (5.3% vs 7.1%), but the difference did not reach statistical significance (p=0.31). Similarly, the prevalences of the C allele were 2.7% and 3.6% for ACS and control groups, respectively (p=0.32). None of the homozygote was detected for the C allele. Further analyses in subjects subgrouped according to sex and age also showed no association of TSP-4 A387P polymorphism with ACS. Furthermore, after adjustment for conventional risk factors by multiple logistic regression analysis, the carrier prevalence of the C allele did not differ significantly between the ACS and control groups (OR=0.85; 95% CI 0.45 to 1.59; p =0.60).
Conclusion The present study suggested that the TSP-4 A387P variant showed a low prevalence compared with western populations and failed to associate with an altered risk of ACS in the Chinese Han population. The findings further supplement experimental data for TSP-4 gene study of the coronary disease.
- gene polymorphism
- acute coronary syndrome