Objective To investigate the effect of Bosentan on carotid artery restenosis in rat and correlation of serum vascular endothelia growth factor (VEGF).
Methods 36 Wistar rats were divided randomly into three groups: control group (n=12), angioplasty group (n=12) and Bosentan (BA) with angioplasty group (n=12). Bosentan was administrated del to rats of BA with angioplasty group. The balloon catheter injury was performed on left common carotid artery of rat by imitating the process of angioplasty. After 7 days and 14 days, neointimal area (NIA) and stenosis index (SI) were calculated. Prior to angioplasty and 7 days after balloon injury, serum VEGF were assessed. In another experiment, 65 Wistar rats were divided randomly into three groups: control group, angioplasty group and Bosentan with angioplasty group. Bosentan was administrated to rats of Bosentan (BA) with angioplasty group. The balloon catheter injury was performed on left common carotid artery of rat by imitating the process of angioplasty. The process of neointimal and media hyperplasia was observed and α-action and PCNA expressions were determined.
Results NIA and SI of BA with angioplasty were significantly decreased compared with angioplasty group (p<0.001). Serum VEGF level significantly increased 7 days after balloon injury both in angioplasty group and BA group. The increase range in BA with angioplasty group was higher than that of the angioplasty group (p<0.01). The serum VEGF level 7 days after injury had linearly negative correlation with SI. In the second experiment, arterial neointima hyperplasia reached delsummit at 28 days in the angioplasty group and 14 days in the BA with angioplasty group. Neointimal and media area of BA with angioplasty at different times (14th, 28th, 45thday) were significantly decreased compared with angioplasty group (p<0.001). The rate of PCNA positive cell increased statistically in BA with angioplasty group than in angioplasty group at 14th day (p<0.01). The rat of α-actin positive cell increased significantly in BA with angioplasty group compared with angioplasty group (p<0.01) at 14th day.
Conclusions Bosentan may be effective on artery restenosis by inhibiting neointimal hyperplasia, increasing the serum VEGF level, reducing the proliferation, migration and transconformation of vascular smooth muscle cells.
- smooth muscle cell
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