Article Text
Abstract
Objective To observe the myocardial protection effects of trimetazidine on Sprague-Dawley (SD) rats with myocardial infarctions (MI).
Methods 90 SD rats were randomly assigned to normal control group (NL, n=30), Trimetazidine group (T, n=30) and sham-operated group (S, n=30). The MI model was set up in SD rats by permanent ligation of the left anterior descending coronary artery. In S group suture was through the left anterior descending coronary artery without ligation. Before and after MI, in NL group/S group and T group normal saline and Trimetazidine (0.3 mg/kg) were separately given by gavage. The changes of serum cTnI were observed at 8, 24, 48 h after MI. The changes of serum cTnI in S group was only observed at 24th hour after operations. In 1 week, 2 weeks and 4 weeks after treatment, the areas of myocardial infarction were analysed, and isovolumic systolic left ventricular maximum rate of pressure rise (+dp/dtmax) and isovolumic diastolic left ventricular maximum rate of pressure drop (−dp/dtmin) were measured to evaluate the myocardial protection effects of STV-1Na. The groups were compared with one-way analysis of variance (ANOVA) test. A value of p 0.05 between NL group and T group. But the serum cTnI level at 24 h after MI decreased in T group (22.7±5.3 ng/ml, p<0.05) compared with NL group (42.3±5.4 ng/ml). The serum cTnI level at 24 h in NL group and T group was significantly increased compared with S group (1.59±1.42 ng/ml) (p<0.01). Trimetazidine (0.248±0.021, p<0.01) decreased significantly the myocardial infarction area compared with NL group (0.362±0.027). The infarction areas in NL group (0.362±0.027) and T group (0.248±0.021) increased significantly compared with S group (0.072±0.1445) (p<0.01). In 1 week after MI, the +dp/dtmax in T group (7535±265) was not significantly different (p>0.05) compared with NL group (6702±329), and the -dp/dtmin in T group (−5511±400) was no significant difference (p>0.05) compared with NL group (−5400±339). In 2 weeks after MI, the +dp/dtmax in T group (8101±313) increased significantly compared with NL group (5868±412) (p<0.01), and the −dp/dtmin in T group (−6514±493) decreased significantly compared with NL group (−4750±463) (p<0.05). In 4 weeks after MI, in T group (7629±374) the +dp/dt max increased significantly compared with NL group (5876±200) (p<0.01,), and the −dp/dtmin in T group (−5883±436) decreased significantly compared with NL group (−4546±279) (p<0.05). The -dp/dt max in T group and NL group were significantly decreased (p<0.05) compared with S group in 1 week, 2 weeks and 4 weeks after the operation. The+dp/dtmin in T group and NL group were increased (p<0.05) compared to S group in 1 week, 2 weeks and 4 weeks after the operation.
Conclusions Trimetazidine has myocardial protection effects on myocardial infarction and improves myocardial systolic and diastolic function in SD rats with acute myocardial infarction.
- Trimetazidine
- myocardial infarction
- cardiac function
- sprague-dawley rat