Objectives The effect of ligustrazine (2,3,5,6-tetramethylpyrazine, TMP) on myocardial ischaemia reperfusion (IR) and the role of nitric oxide (NO) was investigated in a rat IR model.
Methods The animal model was built by ligation of the left anterior descending artery of rats. Sprague-Dawley rats were randomly divided into sham, IR (35 min of regional ischaemia followed by 120 min of reperfusion), 3 TMP pretreated (TMP pretreated, 5, 10, 30 mg/kg intravenous injection, 5 min before ligation) and TMP+L-NAME group. Infarct size, light microscopic evaluation, myocardial superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, myeloperoxidase (MPO) activity, the level of interleukin-1β (IL-1β) as well as NO production were measured.
Results Pretreatment with middle and high dose of TMP markedly decreased infarct size ((38.7±4.5)%, (35.2±2.3)% vs IR(50.4±4.6)%, p<0.05, respectively), increased SOD activity (50.2±3.2 U/mg, 51.2±6.4 U/mg vs IR 35.6±4.3 U/mg, p<0.05, respectively). Middle and high dose of TMP pretreatment decreased MDA content (0.417±0.069 nmol/mg, 0.331±0.023 nmol/mg vs IR 0.693±0.053nmol/mg, p<0.05, respectively) the MPO activity (6.237±0.901 U/mg, 6.201±1.263 U/mg, vs IR 8.670±1.260 U/mg, p<0.05, respectively), and the level of IL-1β (0.140±0.021, 0.132±0.027 pg/mg, vs IR 0.235±0.026 pg/mg, p<0.05). TMP pretreatment also alleviated the neutrophil infiltration and increased the NO level (0.404±0.041 µmol/g in the TMP-M group, 0.453±0.067 µmol/g in the TMP-H group vs 0.295±0.032 µmol/g in the IR group, p<0.05). However, these effects could be significantly reversed by L-NAME which abolished the increase of NO production brought by TMP.
Conclusions In conclusion, ligustrazine attenuated myocardial IR injury by a dose-related manner. The cardioprotective effect of ligustrazine may relate to its effects of increasing the level of NO, decreasing the inflammatory and oxidative stress.
Statistics from Altmetric.com
If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.