PT  - JOURNAL ARTICLE
AU  - G Y Shin
AU  - R J Manuel
AU  - S Ghori
AU  - S Brecker
AU  - A S Breathnach
TI  - Molecular technique identifies the pathogen responsible for culture negative infective endocarditis
AID  - 10.1136/hrt.2004.056762
DP  - 2005 Jun 01
TA  - Heart
PG  - e47--e47
VI  - 91
IP  - 6
4099  - http://heart.bmj.com/content/91/6/e47.short
4100  - http://heart.bmj.com/content/91/6/e47.full
SO  - Heart2005 Jun 01; 91
AB  - A case of culture negative endocarditis complicated by immune complex glomerulonephritis and severe aortic regurgitation necessitated aortic valve replacement. Empirical treatment with penicillin and gentamicin according to UK guidelines was started. The pathogen, Streptococcus sanguis, was later identified by polymerase chain reaction amplification and sequencing of bacterial 16S ribosomal RNA. This molecular technique is likely to be of increasing importance in determining the aetiology of culture negative infective endocarditis, thus providing essential treatment and epidemiological information.