RT Journal Article
SR Electronic
T1 BAS/BSCR21 Akt protects the heart by promoting mitochondrial fusion
JF Heart
JO Heart
FD BMJ Publishing Group Ltd and British Cardiovascular Society
SP e18
OP e18
DO 10.1136/hrt.2010.205781.32
VO 96
IS 17
A1 S B Ong
A1 S Arjun
A1 S Y Lim
A1 S M Davidson
A1 D M Yellon
A1 D J Hausenloy
YR 2010
UL http://heart.bmj.com/content/96/17/e18.2.abstract
AB Background Mitochondria change their morphology by undergoing ‘fusion’ and ‘fission’ to generate elongated and fragmented mitochondria, respectively. We hypothesised that inducing mitochondrial fusion protects the heart against ischaemia-reperfusion injury (IRI), and that this mechanism underlies the cardioprotection elicited by the pro-survival kinase, Akt.Methods/results Inducing mitochondrial fusion in HL-1 cells (a cardiac cell line), using mitochondrial fusion proteins and a pharmacological inhibitor of a mitochondrial fission protein (called mdivi-1) delayed the opening of the mitochondrial permeability transition pore (mPTP, a critical mediator of IRI) and reduced cell death following IRI. Overexpressing Akt induced mitochondrial elongation (49.0±5.8% control vs 72.8±5.0% Akt; N=4 experiments:*p<0.05), delayed mPTP opening (twofold; N=4 experiments: p<0.05); and reduced cell death following IRI (64.9±5.6% control vs 34.2±1.2% Akt; N=4 experiments:*p<0.05). Treatment with the cytokine, erythropoietin (EPO, 10 U/ml), also induced mitochondrial elongation (30.0±3.5% with control vs 67.0±3.4% for EPO; N=4 experiments:*p<0.05), delayed mPTP opening (twofold; N=4 experiments: p<0.05); and reduced cell death following IRI (43.1±2.7% control vs 17.0±2.7% EPO; N=4 experiments:*p<0.05). Finally, elongated mitochondria extending 4–6 μm in length were observed in adult rodent hearts using electron and confocal microscopy. Treatment with mdivi-1 increased the number of elongated mitochondria and protected against IRI, as shown by reduced cell death in adult cardiomyocytes following IRI, and reduced myocardial infarct size in the in vivo murine heart.Conclusions Inducing mitochondrial fusion protects the heart against IRI by delaying mPTP opening. Akt activation also promotes mitochondrial fusion, delays mPTP opening and protects against IRI. These results suggest that modulating mitochondrial morphology may be a novel strategy for cardioprotection.