PT  - JOURNAL ARTICLE
AU  - Quan, He
AU  - Shu, Qin
AU  - Kanghua, Ma
AU  - Suxin, Luo
AU  - Xiaogang, Zhang
TI  - e0044 The role of Ang1 and eNOS in the proangiogenic effect of simvastatin after myocardial infarction in rats
AID  - 10.1136/hrt.2010.208967.44
DP  - 2010 Oct 01
TA  - Heart
PG  - A14--A15
VI  - 96
IP  - Suppl 3
4099  - http://heart.bmj.com/content/96/Suppl_3/A14.4.short
4100  - http://heart.bmj.com/content/96/Suppl_3/A14.4.full
SO  - Heart2010 Oct 01; 96
AB  - Objective To investigate the roles of angiopoietin-1 (Ang-1) and endothelial nitric oxide synthase (eNOS) in pro-angiogenic effect of simvastatin after experimental myocardial infarction (MI). Methods 60 healthy adult SD rats were randomly divided into the sham operated group, control group, simvastatin group, simvastatin plus L-NAME (inhibitor of NOS) group and simvastatin plus AMG386 (inhibitor of Ang-1) group; Left anterior descending coronary was undergone permanent occlusion to establish the MI model. Rats with MI were administered simvastatin (1 mg/(kg·d)), simvastatin plus L-NAME (40 mg/(kg·d)), and simvastatin plus AMG386 (10 mg/(kg·wk)) respectively for 2 weeks. New microvessels in the ischaemic area near the infarction myocardium were stained by CD31 and the density of new microvessels was dedected; Ang-1, eNOS and phosphoralated endothelial nitric oxide synthase at Ser1177 (p-eNOS) were evaluated by western blotting and RT-PCR assay. Results (1) simvastatin significantly increased the density of new microvessels (p<0.05), but L-NAME and AMG386 significantly inhibited the pro-angiogenic effect of simvastatin (p<0.05). (2) simvastatin significantly improved The expression of Ang-1, eNOS and p-eNOS (p<0.05), and AMG386 significantly decreased simvastatin induced upregulation of p-eNOS. Conclusion The pro-angiogenic effect of simvastatin is associated with increased expression of Ang-1, eNOS and p-eNOS, and phosphoralation of eNOS maybe the downstream pathway for Ang-1 induced angiogenesis.