RT Journal Article
SR Electronic
T1 44 Tiling array shows low ANRIL, high CDKN2B expression associated with chromosome 9p21 coronary artery disease (CAD) risk genotype
JF Heart
JO Heart
FD BMJ Publishing Group Ltd and British Cardiovascular Society
SP e7
OP e7
DO 10.1136/heartjnl-2011-300920b.44
VO 97
IS 20
A1 L D Beeton
A1 P Chivers
A1 J Dawes
A1 T Kyriakou
A1 A Goel
A1 J Peden
A1 F R Green
YR 2011
UL http://heart.bmj.com/content/97/20/e7.46.abstract
AB Recent studies suggest that ANRIL expression mediates susceptibility to CAD1 via CDKN2B.2 We used fluorescently-labelled whole-blood RNA, from 20 healthy volunteers genotyped for the CAD-risk-SNP rs2891168, to probe custom-designed Agilent tiling expression microarrays. Raw data were normalized to probe GC content and housekeeping genes. We found that ANRIL exons 1–4 were more abundantly expressed in blood than 5–20, with exons 6, 8, 9, 20 showing low expression. We derived a set of “training” tiling probes from HUVEC cells in which ANRIL expression was attenuated using siRNA against exons 13 and 19. ANRIL expression (probes in exons 5,6,8,13,16,18,19) was reduced by at least 50% and CDKN2B expression (probes in exons 1,2) increased, with no effect on CDKN2A. We confirmed these data using real-time QPCR. Using the tiling probe “training-set”, a probe in exon 16 of ANRIL showed a CAD genotype-specific difference in expression (p<0.001), with the risk-allele lower, and probes in exon 2 of CDKN2B showed higher expression for the risk-allele (p<0.05 and p<0.01). In conclusion, we found by a novel technique that ANRIL expression in whole-blood was CAD-risk-genotype-specific, and confirmed that low ANRIL was correlated with higher CDKN2B, but not CDKN2A expression. These data support recent studies implicating reduced ANRIL expression and reciprocal over-expression of CDKN2B, which is thought to impair TGFβ signalling, in CAD susceptibility.